Oral administration of whole dihomo-γ-linolenic acid-producing yeast suppresses allergic contact dermatitis in mice.
Oral administration of whole dihomo-γ-linolenic acid-producing yeast suppresses allergic contact dermatitis in mice.
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口服全二高-γ-亚麻酸生产酵母可抑制小鼠过敏性接触性皮炎。
DOI：
10.1080/09168451.2019.1667220
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
H
中科院分区：
文献类型：
--
作者：
Watanabe;N.;Teradu;S.;Ohtani;M.;Uemura;H
已知二高-γ-亚麻酸（DGLA，C20：3 n-6）具有抗炎活性，但由于其有限的天然来源，其作用范围尚未得到充分研究。我们通过引入两个去饱和酶基因和一个延伸酶基因将内源油酸转化为DGLA，构建了一株具有完整DGLA合成代谢途径的酵母菌株，解决了这些问题。利用S.为了研究热灭活的产DGLA的酵母细胞对刺激性接触性皮炎的作用，我们先前研究了热灭活的产DGLA的酵母细胞对刺激性接触性皮炎的作用，并且表明口服摄入这种酵母显著抑制炎症反应，而摄入25倍量的纯化DGLA没有观察到这种抑制。由于这种方法被认为是一种简单有效的抑制炎症的方法，我们在这项研究中检查了它对过敏性接触性皮炎（ACD）的有效性，并表明这种方法对ACD也有效。
Dihomo-γ-linolenic acid (DGLA, C20: 3n-6) is known to have an anti-inflammatory activity, but its range of effects was not well studied because of its limited natural sources. We addressed these issues by constructing an yeastSaccharomyces cerevisiaestrain having a complete metabolic pathway for DGLA synthesis by introducing two desaturase and one elongase genes to convert endogenous oleic acid to DGLA. Taking advantage of well-known safety ofS. cerevisiae, we previously investigated the efficacy of heat-killed whole DGLA-producing yeast cells on irritant contact dermatitis, and showed that oral intake of this yeast significantly suppressed inflammatory reactions, whereas no such suppression was observed by the intake of 25 times the amount of purified DGLA. Since this method is considered to be a simple and efficient way to suppress inflammation, we examined its effectiveness against allergic contact dermatitis (ACD) in this study and showed that this method was also effective against ACD.