Ultra-fast multispectral optical imaging of cortical oxygenation, blood flow, and intracellular calcium dynamics.
Ultra-fast multispectral optical imaging of cortical oxygenation, blood flow, and intracellular calcium dynamics.
复制标题
DOI：
10.1364/oe.17.015670
复制
发表时间：
2009-08-31
期刊：
影响因子：
3.8
通讯作者：
Hillman EM
中科院分区：
文献类型：
--
作者：
Bouchard MB;Chen BR;Burgess SA;Hillman EM
暴露的大脑的基于相机的光学成像允许检查皮质对刺激的血液动力学反应。典型的多光谱成像系统利用照相机和几个波长的照明，允许区分氧合血红蛋白和脱氧血红蛋白浓度的变化。然而，大多数多光谱成像系统利用白色光源和机械滤光轮来复用照明波长，这是缓慢的并且难以在高帧速率下同步。我们提出了一种新的基于LED的系统，能够在超过220 Hz的帧速率的高分辨率多光谱成像。这种改进的性能使得能够同时可视化单个血管内的血红蛋白氧合动力学、血管直径的变化、来自红细胞运动的血流动力学以及动态变化的荧光。
Camera-based optical imaging of the exposed brain allows cortical hemodynamic responses to stimulation to be examined. Typical multispectral imaging systems utilize a camera and illumination at several wavelengths, allowing discrimination between changes in oxy- and deoxyhemoglobin concentration. However, most multispectral imaging systems utilize white light sources and mechanical filter wheels to multiplex illumination wavelengths, which are slow and difficult to synchronize at high frame rates. We present a new LED-based system capable of high-resolution multispectral imaging at frame rates exceeding 220 Hz. This improved performance enables simultaneous visualization of hemoglobin oxygenation dynamics within single vessels, changes in vessel diameters, blood flow dynamics from the motion of erythrocytes, and dynamically changing fluorescence.