Boron Accelerates Cultured Osteoblastic Cell Activity through Calcium Flux
Boron Accelerates Cultured Osteoblastic Cell Activity through Calcium Flux
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DOI：
10.1007/s12011-016-0719-y
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发表时间：
2016-12-01
影响因子：
3.9
通讯作者：
Hayashi, Yoshihiko
中科院分区：
文献类型：
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作者：
Capati, Mark Luigi Fabian;Nakazono, Ayako;Hayashi, Yoshihiko
低浓度的硼(B)可促进哺乳动物成骨细胞的增殖和分化。本研究旨在探讨0.1 mM的B对体外培养的成骨细胞膜功能的影响。通过基因表达微阵列分析研究与细胞活性有关的基因。钙内流和外流的检测显示，钙内流由L型钙通道激活，钙流出由Na+/K+-ATPase激活。实时荧光定量聚合酶链式反应分析表明，在添加B的培养液中培养3d后，4个矿化相关基因的信使RNA(MRNAs)表达明显增加。利用基因芯片分析，与对照组相比，与细胞增殖和分化有关的五个基因上调。在钙内流方面，硝苯地平组加入B液后1min的相对荧光强度与加入前1min相比无明显增加。对照组相对荧光强度较实验组显著增强(P<；0.05)。在钙外流方面，在0.1 mM添加B的培养液中，哇巴因处理10min的相对荧光强度的斜率值明显低于对照组(P<；0.01)。本研究首次证实了补B对成骨细胞内钙离子流动的促进作用。细胞膜的稳定性与极低浓度的B在体外促进哺乳动物成骨细胞增殖和分化的机制有关。
A low concentration of boron (B) accelerates the proliferation and differentiation of mammalian osteoblasts. The aim of this study was to investigate the effects of 0.1 mM of B on the membrane function of osteoblastic cells in vitro. Genes involved in cell activity were investigated using gene expression microarray analyses. The Ca2+ influx and efflux were evaluated to demonstrate the activation of L-type Ca2+ channel for the Ca2+ influx, and that of Na+/K+-ATPase for the Ca2+ efflux. A real-time PCR analysis revealed that the messenger RNA (mRNA) expression of four mineralization-related genes was clearly increased after 3 days of culture with a B-supplemented culture medium. Using microarray analyses, five genes involved in cell proliferation and differentiation were upregulated compared to the control group. Regarding the Ca2+ influx, in the nifedipine-pretreated group, the relative fluorescence intensity for 1 min after adding B solution did not increase compared with that for 1 min before addition. In the control group, the relative fluorescence intensity was significantly increased compared with the experimental group (P < 0.05). Regarding the Ca2+ efflux, in the experimental group cultured in 0.1 mM of B-supplemented medium, the relative fluorescence intensity for 10 min after ouabain treatment revealed a significantly lower slope value compared with the control group (P < 0.01). This is the first study to demonstrate the acceleration of Ca2+ flux by B supplementation in osteoblastic cells. Cell membrane stability is related to the mechanism by which a very low concentration of B promotes the proliferation and differentiation of mammalian osteoblastic cells in vitro.