Generation of Mercury-Hyperaccumulating Plants through Transgenic Expression of the Bacterial Mercury Membrane Transport Protein MerC
Generation of Mercury-Hyperaccumulating Plants through Transgenic Expression of the Bacterial Mercury Membrane Transport Protein MerC
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DOI：
10.1007/s11248-006-9008-4
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发表时间：
2006-07
影响因子：
3
通讯作者：
Y. Sasaki;T. Hayakawa;C. Inoue;A. Miyazaki;S. Silver;T. Kusano
中科院分区：
文献类型：
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作者：
Y. Sasaki;T. Hayakawa;C. Inoue;A. Miyazaki;S. Silver;T. Kusano
来自嗜酸氧化亚铁硫杆菌的C基因具有吸汞泵的功能。MerC蛋白定位于植物细胞的细胞质膜。将花椰菜花叶病毒35 S启动子控制下的merC基因导入拟南芥和烟草植株中，结果发现merC基因的过量表达使宿主植物对Hg ~（2+）高度敏感，其积累的Hg ~（2+）离子量约为野生型植株的两倍。因此，细菌汞离子转运蛋白，如MerC可能是有用的分子工具，生产转基因植物，超积累Hg 2+离子。
ThemerCgene fromAcidithiobacillus ferrooxidansfunctions as a mercury uptake pump. MerC protein localizes in the cytoplasmic membrane of plant cells. WhenArabidopsis thalianaand tobacco plants were transformed with themerCgene under the control of theCauliflower mosaic virus35S promoter, the resulting overexpression ofmerCrendered the host plants hypersensitive to Hg2+and they accumulated approximately twice as much Hg2+ion as the wild type plants. Thus, bacterial mercuric ion transporters such as MerC may be useful molecular tools for producing transgenic plants that hyperaccumulate Hg2+ion.