Well-Controlled Cell-Trapping Systems for Investigating Heterogeneous Cell-Cell Interactions
Well-Controlled Cell-Trapping Systems for Investigating Heterogeneous Cell-Cell Interactions
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用于研究异质细胞间相互作用的控制良好的细胞捕获系统
DOI：
10.1002/adhm.201701208
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发表时间：
2018
影响因子：
10
通讯作者：
Takeuchi Shoji
中科院分区：
文献类型：
--
作者：
Kamiya Koki;Abe Yuta;Inoue Kosuke;Osaki Toshihisa;Kawano Ryuji;Miki Norihisa;Takeuchi Shoji
微流体系统已被开发用于对单细胞进行图案化以研究细胞与细胞的相互作用。然而，对多种类型的细胞进行图案化以理解异质细胞与细胞之间的相互作用仍然很困难。在这里，目的是开发一种细胞捕获装置，以良好控制的顺序和形态组装多种类型的细胞。该装置主要包括用于组装细胞的聚对二甲苯片和用于控制细胞捕获区域的微梳。通过使用镊子在 SU-8 微梳上移动聚对二甲苯片来控制细胞捕获区域。温和的向下流动用作细胞捕获的驱动力。演示了在具有圆形和线形孔的聚对二甲苯片上组装电池。然后通过连接蛋白纳米孔直接细胞间转移钙黄绿素来研究被捕获细胞的细胞间接触。最后，使用带有控制细胞捕获区域的系统的设备，以良好控制的顺序组装三种不同类型的细胞。使用该装置获得的正确细胞排序率为27.9%，高于没有使用滑动聚对二甲苯系统时获得的正确细胞排序率（0.74%）。此外，还验证了组装的三种细胞类型之间细胞与细胞接触的发生。这种细胞图案化装置将成为研究异质细胞间相互作用的有用工具。
Microfluidic systems have been developed for patterning single cells to study cell–cell interactions. However, patterning multiple types of cells to understand heterogeneous cell–cell interactions remains difficult. Here, it is aimed to develop a cell‐trapping device to assemble multiple types of cells in the well‐controlled order and morphology. This device mainly comprises a parylene sheet for assembling cells and a microcomb for controlling the cell‐trapping area. The cell‐trapping area is controlled by moving the parylene sheet on an SU‐8 microcomb using tweezers. Gentle downward flow is used as a driving force for the cell‐trapping. The assembly of cells on a parylene sheet with round and line‐shaped apertures is demonstrated. The cell–cell contacts of the trapped cells are then investigated by direct cell–cell transfer of calcein via connexin nanopores. Finally, using the device with a system for controlling the cell‐trapping area, three different types of cells in the well‐controlled order are assembled. The correct cell order rate obtained using the device is 27.9%, which is higher than that obtained without the sliding parylene system (0.74%). Furthermore, the occurrence of cell–cell contact between the three cell types assembled is verified. This cell‐patterning device will be a useful tool for investigating heterogeneous cell–cell interactions.