Universal primers for the PCR-mediated amplification of DNA 1:: A satellite-like molecule associated with begomovirus-DNA β complexes
Universal primers for the PCR-mediated amplification of DNA 1:: A satellite-like molecule associated with begomovirus-DNA β complexes
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DOI：
10.1385/mb:23:1:83
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发表时间：
2003-01-01
影响因子：
2.6
通讯作者：
Markham, PG
中科院分区：
文献类型：
--
作者：
Bull, SE;Briddon, RW;Markham, PG
DNA 1是约1370个核苷酸的单链DNA分子。它与双生病毒属的单分裂双生病毒相关，其需要DNA β组分用于症状感染。DNA 1分子在植物中的运动需要辅助性菜豆花叶病毒，但能够自我复制。我们针对DNA 1的保守序列设计了两对邻接引物（DNA 101/DNA 102和UN 101/UN 102）。这允许聚合酶链反应介导的扩增全长分子的总核酸提取物产生的各种宿主植物从地理上不同的，世界各地的位置。这些引物既可用作诊断探针，又可用于产生用于植物体内研究的全长感染性克隆。
DNA 1 is a single-stranded DNA molecule of approximately 1370 nucleotides. It is associated with monopartite geminiviruses of the genus Begomovirus, which require a DNA beta component for symptomatic infection. The DNA 1 molecule requires the helper begomovirus for movement in plants, but is capable of self-replication. We designed two abutting primer pairs (DNA101/DNA102 and UN101/UN102) to conserved sequences of DNA 1. This allowed polymerase chain reaction-mediated amplification of the full-length molecule from total nucleic acid extracts produced from various host plants from geographically distinct, worldwide locations. These primers are useful both as diagnostic probes and for producing full-length infectious clones for in planta studies.