Controlled Neighbor Exchanges Drive Glassy Behavior, Intermittency, and Cell Streaming in Epithelial Tissues
Controlled Neighbor Exchanges Drive Glassy Behavior, Intermittency, and Cell Streaming in Epithelial Tissues
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DOI：
10.1103/physrevx.11.041037
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发表时间：
2021-11-22
影响因子：
12.5
通讯作者：
Bi, Dapeng
中科院分区：
文献类型：
--
作者：
Das, Amit;Sastry, Srikanth;Bi, Dapeng
细胞邻居交换是生物学中组织重排的组成部分，包括发育和修复。通常，这些过程通过拓扑T1转变发生，类似于在泡沫、颗粒和胶体中观察到的那些。然而，与非生命材料中的T1跃迁相反，生物组织中的T1跃迁是速率受限的，并且由于在细胞-细胞连接处重塑复杂结构所需的有限时间而不能瞬时发生。在这里，我们研究了这种限速过程如何影响力学和集体行为的细胞在组织中引入这个重要的生物学约束在一个理论的基于顶点的模型作为一个内在的单细胞属性。我们报告说，在没有这个时间限制，组织经历了玻璃化转变与细胞运动性降低的特点是急剧增加的细胞-细胞重排的不稳定性。值得注意的是，这种玻璃化转变消失了，因为T1转变是暂时有限的。作为有限重排的独特结果，我们还发现组织发展出空间相关的快细胞和慢细胞群体，其中快细胞组织成流状模式，并保持最佳稳定的细胞-细胞接触。这项工作的预测与现有的果蝇蛹发育的体内实验进行了比较。
Cell neighbor exchanges are integral to tissue rearrangements in biology, including development and repair. Often, these processes occur via topological T1 transitions analogous to those observed in foams, grains, and colloids. However, in contrast to those in nonliving materials, the T1 transitions in biological tissues are rate limited and cannot occur instantaneously due to the finite time required to remodel complex structures at cell-cell junctions. Here, we study how this rate-limiting process affects the mechanics and collective behavior of cells in a tissue by introducing this important biological constraint in a theoretical vertex-based model as an intrinsic single-cell property. We report that, in the absence of this time constraint, the tissue undergoes a glass transition with lowering of cell motility characterized by a sharp increase in the intermittency of cell-cell rearrangements. Remarkably, this glass transition disappears, as T1 transitions are temporally limited. As a unique consequence of limited rearrangements, we also find that the tissue develops spatially correlated populations of fast and slow cells, in which the fast cells organize into streamlike patterns and maintain optimally stable cell-cell contacts. The predictions of this work are compared with existing in vivo experiments in Drosophila pupal development.