Nonporous polyurethane membranes as islet immunoisolation matrices - Biocompatibility studies
Nonporous polyurethane membranes as islet immunoisolation matrices - Biocompatibility studies
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DOI：
10.1106/088532802024249
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发表时间：
2002-04-01
影响因子：
2.9
通讯作者：
Bhonde, RR
中科院分区：
文献类型：
--
作者：
George, S;Nair, PD;Bhonde, RR
合成了具有不同硬段含量的新型弹性体无孔聚氨酯膜，用于评估可能的胰岛包封基质。这些膜的物理化学性质已由作者较早报道，并已被发现适用于免疫隔离。在本研究中，评价了膜的体外生物相容性。在对L929成纤维细胞的直接细胞接触研究中，膜T1、T4、T5和T6未显示出毒性。但是，发现T2和T3具有细胞毒性，因此从进一步试验中排除。MTT法证实T4、T5和T6的浸出产物对NIH3T3细胞无细胞毒性，而T1则降低细胞活力。单独的T4和T5与小鼠胰岛相容，而T6与小鼠胰岛不相容。数字图像分析（DIA）研究显示，在T4和T5上培养的胰岛形态完整，活力（分别为88.4%和91%）与组织培养聚苯乙烯（TOPS）对照上的胰岛相当。T4和T5上的胰岛也保留了它们的功能，如通过响应于体外葡萄糖挑战（16.0 mM）的胰岛素分泌所判断的。这些研究指出了决定聚氨酯膜与胰岛相容性的表面自由能和亲水性的关键作用。研究表明，聚氨酯膜T4和T5可能是胰岛免疫隔离的潜在候选者。
Novel elastomeric nonporous polyurethane membranes were synthesised with differing hard segment contents for evaluation as possible islet encapsulation matrices. Physico-chemical properties of these membranes were reported earlier by authors and have been found suitable for immunoisolation. In the present study, membranes were evaluated for their in vitro biocompatibility. Membranes T1, T4, T5 and T6 did not show toxicity in direct cell contact study towards L929 fibroblasts. However, T2 and T3 were found cytotoxic and were excluded from further testing. NIH3T3 cells when exposed to leach out products of T4, T5 and T6 showed no cytotoxicity, while T1 decreased cellular viability as confirmed by MTT assay. T4 and T5 alone were seen to be compatible with mouse islets while T6 was incompatible to the mouse islets. Digital image analysis (DIA) studies showed intact morphology of islets cultured on the T4 and T5 with viability (88.4 and 91% respectively) comparable to islets on tissue culture polystyrene (TOPS) control. Islets on T4 and T5 also retained their functionality, as judged by insulin secretion in response to in vitro glucose challenge (16.0 mM). These studies point out the crucial role of surface free energy and hydrophilicity in deciding compatibility of polyurethane membranes with islets of Langerhans. Studies indicate that polyurethane membranes T4 and T5 could be potential candidates for islet immunoisolation.