Overexpression of the Saussurea medusa chalcone isomerase gene in Saussurea involucrata hairy root cultures enhances their biosynthesis of apigenin
Overexpression of the Saussurea medusa chalcone isomerase gene in Saussurea involucrata hairy root cultures enhances their biosynthesis of apigenin
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雪莲毛状根培养物中水母雪莲查尔酮异构酶基因的过度表达增强了芹菜素的生物合成
DOI：
10.1016/j.phytochem.2005.12.004
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发表时间：
2006-03-01
期刊：
影响因子：
3.8
通讯作者：
Ma, FS
中科院分区：
文献类型：
--
作者：
Li, FX;Jin, ZP;Ma, FS
雪莲是一种药用植物，以其类黄酮而闻名，其中包括芹菜素，已被证明可以显着抑制肿瘤发生。由于天然存在的芹菜素丰度非常低，我们采用转基因方法通过改造类黄酮途径来增加芹菜素产量。构建了包含受花椰菜花叶病毒 (CaMV) 35S 启动子控制的雪莲水母查尔酮异构酶 (CHI) 基因的完整 cDNA 序列的构建体。使用发根农杆菌介导的转化系统，将 chi 过表达盒整合到珙桐基因组中，并建立了转基因毛状根系。 CHI 将柚皮素查尔酮转化为柚皮素，柚皮素是芹菜素的前体。我们观察到，转基因毛状根系比野生型毛状根生长更快，产生更高水平的芹菜素和总黄酮类化合物。经过 5 周的培养期，表现最好的品系 (C46) 积累了 32.1 mg L-1 芹菜素和 647.8 mg L-1 总黄酮类化合物，分别比野生型毛状根高 12 倍和 4 倍。生产率的提高与 CHI 活性的提高相对应，证实了 CHI 在总黄酮和芹菜素合成中发挥的关键作用以及当前代谢工程策略的效率。 (c) 2005 Elsevier Ltd. 保留所有权利。
Saussurea involucrata is a medicinal plant well known for its flavonoids, including apigenin, which has been shown to significantly inhibit tumorigenesis. Since naturally occurring apigenin is in very low abundance, we took a transgenic approach to increase apigenin production by engineering the flavonoid pathway. A construct was made to contain the complete cDNA sequence of the Saussurea medusa chalcone isornerase (CHI) gene under the control of the cauliflower mosaic virus (CaMV) 35S promoter. Using an Agrobacterium rhizogenes-mediated transformation system, the chi overexpression cassette was incorporated into the genome of S. involucrata, and transgenic hairy root lines were established. CHI converts naringenin chalcone into naringenin that is the precursor of apigenin. We observed that transgenic hairy root lines grew faster and produced higher levels of apigenin and total flavonoids than wild-type hairy roots did. Over a culture period of 5 weeks, the best-performing line (C46) accumulated 32.1 mg L-1 apigenin and 647.8 mg L-1 total flavonoids, or 12 and 4 times, respectively, higher than wild-type hairy roots did. The enhanced productivity corresponded to elevated CHI activity, confirming the key role that CHI played for total flavonoids and apigenin synthesis and the efficiency of the current metabolic engineering strategy. (c) 2005 Elsevier Ltd. All rights reserved.