PRODUCTION OF TRANSGENIC RICE (ORYZA-SATIVA L) PLANTS FROM AGRONOMICALLY IMPORTANT INDICA AND JAPONICA VARIETIES VIA ELECTRIC-DISCHARGE PARTICLE-ACCELERATION OF EXOGENOUS DNA INTO IMMATURE ZYGOTIC EMBRYOS
PRODUCTION OF TRANSGENIC RICE (ORYZA-SATIVA L) PLANTS FROM AGRONOMICALLY IMPORTANT INDICA AND JAPONICA VARIETIES VIA ELECTRIC-DISCHARGE PARTICLE-ACCELERATION OF EXOGENOUS DNA INTO IMMATURE ZYGOTIC EMBRYOS
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DOI：
10.1038/nbt1091-957
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发表时间：
1991-10-01
期刊：
影响因子：
--
通讯作者：
KOFRON, M
中科院分区：
文献类型：
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作者：
CHRISTOU, P;FORD, TL;KOFRON, M
我们已经恢复了转基因水稻植物从一些商业上重要的品种，包括到目前为止，籼稻品种，使用放电粒子加速。 用携带DNA的金颗粒轰击来自温室生长的植物的不成熟胚，并按照简单的培养方案回收转基因植物。 外源基因在R1代植株中呈孟德尔分离，Southern杂交分析表明外源基因在R1代植株中稳定整合。 替代的转化方案，是依赖于原生质体和悬浮培养系统的发展不再是必要的，因为我们已经表明，各种各样的不同品种可以转化。 已经获得了表达除草剂抗性等农艺学有用性状的转基因植物，目前正在进行进一步评估。 本报告还表明，它是可能的，通过转化未成熟胚的盾叶组织产生转基因单子叶植物。
We have recovered transgenic rice plants from a number of commercially important cultivars, including until now recalcitrant Indica varieties, using electric discharge particle acceleration. Immature embryos from greenhouse-grown plants were bombarded with gold particles carrying DNA, and transgenic plants were recovered following a simple culture protocol. Mendelian segregation of foreign genes was observed in R1 progeny and stable integration was demonstrated by Southern blot analysis of genomic DNA isolated from progeny plants. Alternative transformation protocols that are dependent on the development of protoplast and suspension culture systems are no longer necessary as we have shown that a wide variety of diverse cultivars can be transformed. Transgenic plants expressing agronomically useful traits such as herbicide resistance have been obtained and are currently undergoing further evaluation. This report also demonstrates that it is possible to produce transgenic monocoty-ledonous plants by transforming scutellar tissue of immature embryos.