Mass spectrometric analysis of neutral sphingolipids: Methods, applications, and limitations
Mass spectrometric analysis of neutral sphingolipids: Methods, applications, and limitations
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DOI：
10.1016/j.bbalip.2011.05.011
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发表时间：
2011-11-01
影响因子：
4.8
通讯作者：
Sandhoff, Konrad
中科院分区：
文献类型：
--
作者：
Farwanah, Hany;Kolter, Thomas;Sandhoff, Konrad
鞘磷脂是脂类中的重要一类，尤其是考虑到它们在脂类代谢中的重要作用。因此，已经创造了各种方法来完成它们的分析，最近创造了术语“鞘脂组学”，以强调能够对所有鞘脂物种进行全面分析的动机，包括酸性和中性物种。在这篇综述中，我们总结了一些主要以生物医学为基础的分析中性鞘磷脂的质谱学方法的优点、应用和局限性。为了强调方法开发的一些实际方面，我们重点介绍了我们实验室最近开发的一种新方法，该方法可以一次完成神经酰胺、己糖神经酰胺、乳糖神经酰胺、球三糖神经酰胺、球四糖神经酰胺、鞘磷脂物种和胆固醇的分离、检测和质谱分析。该方法可用于研究神经鞘脂病等疾病的中性鞘磷脂代谢的损害，并可用于筛选由基因敲除实验或相关研究引起的鞘磷脂谱变化。这篇文章是题为：脂代谢与成像质谱学的特刊的一部分。(C)2011爱思唯尔B.V.保留所有权利。
Sphingolipids represent an important class among lipids, especially when considering their vital roles in lipid metabolism. Thus, a variety of methods have been created to accomplish their analysis and the term "sphingolipidomics" has recently been coined to underline the motivation to enable a comprehensive analysis of all sphingolipid species including the acidic and the neutral ones. In this review, we summarize selected mainly biomedical based mass spectrometric approaches for the analysis of neutral sphingolipids regarding their advantages, applications and limitations. To underline some practical aspects of method development, we focus on a new method recently developed in our laboratory, which enables separation, detection, and mass spectrometric profiling of ceramide, hexosylceramide, lactosylceramide, globotriaosylceramide, globotetraosylceramide, sphingomyelin species, and cholesterol in one run. This method can be applied to investigate impairments of neutral sphingolipid metabolism in a variety of disorders such as sphingolipidoses and be employed to screen for sphingolipid profile changes as induced by knockout experiments or related studies. This article is part of a Special Issue entitled: Lipodomics and Imaging Mass Spectrometry. (C) 2011 Elsevier B.V. All rights reserved.