Identification of organic anion transporting polypeptide 4 (Oatp4) as a major full-length isoform of the liver-specific transporter-1 (rlst-1) in rat liver
Identification of organic anion transporting polypeptide 4 (Oatp4) as a major full-length isoform of the liver-specific transporter-1 (rlst-1) in rat liver
复制标题
DOI：
10.1016/s0014-5793(00)01596-9
复制
发表时间：
2000-06-02
期刊：
影响因子：
3.5
通讯作者：
Meier, PJ
中科院分区：
文献类型：
--
作者：
Cattori, V;Hagenbuch, B;Meier, PJ
从大鼠肝脏中分离到一种新的有机阴离子转运多肽（Oatp）4(1)，该多肽比报道的大鼠肝脏特异性有机阴离子转运蛋白(rlst)-1长35个氨基酸，与人类otp - c （LST-1/OATP2；基因符号SLC21A6）具有64%的氨基酸序列同源性。当在非洲爪鼠卵母细胞中表达时，Oatp4 （Slc21a10）介导多种有机阴离子的多特异性摄取，包括牛磺酸胆酸盐（K(m)类似于27 μ m）、溴磺酰眼蛋白（K(m)类似于1.1 μ m）和类固醇偶联物。基于核酸酶保护分析，Oatp4似乎是大鼠肝脏中主要的转录物，表明rlst-1在肝脏整体有机阴离子摄取中起次要作用。(C) 2000年欧洲生化学会联合会。Elsevier Science B.V.版权所有。
A novel organic anion transporting polypeptide (Oatp)4(1) was isolated from rat liver that is 35 amino acids longer than the reported rat liver specific organic anion transporter (rlst)-1 and exhibits a 64% amino acid sequence identity with the human OATP-C (LST-1/OATP2; gene symbol SLC21A6). When expressed in Xenopus laevis oocytes, Oatp4 (Slc21a10) mediated polyspecific uptake of a variety of organic anions including taurocholate (K(m)similar to 27 mu M), bromosulfophthalein (K(m)similar to 1.1 mu M) and steroid conjugates, Based on nuclease protection analysis Oatp4 appears to be the predominant transcript in rat liver indicating that rlst-1 plays a minor role in overall hepatic organic anion uptake. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.