KAP-1, a novel corepressor for the highly conserved KRAB repression domain
KAP-1, a novel corepressor for the highly conserved KRAB repression domain
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DOI：
10.1101/gad.10.16.2067
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发表时间：
1996-08-15
影响因子：
10.5
通讯作者：
Rauscher, FJ
中科院分区：
文献类型：
--
作者：
Friedman, JR;Fredericks, WJ;Rauscher, FJ
KRAB抑制结构域是迄今为止发现的分布最广泛的转录效应结构域之一，但其抑制机制尚不清楚。我们已经克隆了一个辅阻遏物，KAP-1，它与KRAB结构域，但不与KRAB突变体，失去了抑制活性。KAP-1可以增强KRAB介导的阻遏，并且当直接连接到DNA时是阻遏物。KAP-1含有一个RING指、B盒和一个PHD指; RING-B1-B2结构是KRA B结合和共阻遏所必需的。我们认为KAP-1可能是含KRAB结构域转录因子大家族的通用辅抑制子。
The KRAB repression domain is one of the most widely distributed transcriptional effector domains yet identified, but its mechanism of repression is unknown. We have cloned a corepressor, KAP-1, which associates with the KRAB domain but not with KRAB mutants that have lost repression activity. KAP-1 can enhance KRAB-mediated repression and is a repressor when directly tethered to DNA. KAP-1 contains a RING finger, B boxes, and a PHD finger; the RING-B1-B2 structure is required for KRAB binding and corepression. We propose that KAP-1 may be a universal corepressor for the large family of KRAB domain-containing transcription factors.