Deletion of MUD2, the yeast homolog of U2AF65, can bypass the requirement for Sub2, an essential spliceosomal ATPase
Deletion of MUD2, the yeast homolog of U2AF65, can bypass the requirement for Sub2, an essential spliceosomal ATPase
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DOI：
10.1101/gad.851301
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发表时间：
2001-01-01
影响因子：
10.5
通讯作者：
Guthrie, C
中科院分区：
文献类型：
--
作者：
Kistler, AL;Guthrie, C
哺乳动物的U2AF65和UAP56是形成桥前小体(PS)所必需的。我们检验了酵母UAP56同源物，subs是同一步骤所需的预测，并与U2AF65的酵母同源物MUDS协同作用。出乎意料的是，2比1。萃取物积累类PS复合体。此外，MUD2的缺失加剧了sub2等位基因的cs表型，但同时抑制了is sub2-1和致死性Delta sub2的表型。我们认为，在PS形成前后，Sub2与Mud2在功能上都相互作用。在没有Mud2的情况下，Sub2函数变得可有可无。
Mammalian U2AF65 and UAP56 are required for prespliceosome (PS) formation. We tested the predictions that the yeast UAP56 homolog, SUBS is required for the same step and functions collaboratively with MUDS the yeast homolog of U2AF65. Unexpectedly, sub2-1. extracts accumulate PS-like complexes. Moreover, deletion of MUD2 exacerbates the cs phenotype of sub2 alleles yet suppresses both the is sub2-1 and the lethal Delta sub2 phenotypes. We propose that Sub2 functionally interacts with Mud2 both before and after PS formation. In the absence of Mud2, Sub2 function becomes dispensable.