Mutations in human dynamin block an intermediate stage in coated vesicle formation.
Mutations in human dynamin block an intermediate stage in coated vesicle formation.
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DOI：
10.1083/jcb.122.3.553
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发表时间：
1993-08
期刊：
影响因子：
--
通讯作者：
Schmid SL
中科院分区：
文献类型：
--
作者：
van der Bliek AM;Redelmeier TE;Damke H;Tisdale EJ;Meyerowitz EM;Schmid SL
通过哺乳动物细胞中 GTP 结合域突变体的瞬时表达，研究了人类动力在受体介导的内吞作用中的作用。使用检测包被囊泡形成中的中间体的测定，发现动力突变体在包被组装开始之后和配体被隔离到深度内陷的包被凹坑中之前的阶段阻断内吞作用。从内质网到高尔基复合体的膜转运不受影响，表明动力突变体特异性阻断内吞作用的早期事件。这些结果表明，动力蛋白 GTP 结合域的突变会阻断哺乳动物细胞中的 Tfn 内吞作用，并表明功能性动力 GTP 酶是通过网格蛋白包被的凹坑进行受体介导的内吞作用所必需的。
The role of human dynamin in receptor-mediated endocytosis was investigated by transient expression of GTP-binding domain mutants in mammalian cells. Using assays which detect intermediates in coated vesicle formation, the dynamin mutants were found to block endocytosis at a stage after the initiation of coat assembly and preceding the sequestration of ligands into deeply invaginated coated pits. Membrane transport from the ER to the Golgi complex was unaffected indicating that dynamin mutants specifically block early events in endocytosis. These results demonstrate that mutations in the GTP-binding domain of dynamin block Tfn-endocytosis in mammalian cells and suggest that a functional dynamin GTPase is required for receptor-mediated endocytosis via clathrin-coated pits.