Genetic control of immune responses in vitro. I. Development of primary and secondary plaque-forming cell responses to the random terpolymer 1-glutamic acid 60-1-alanine30-1-tyrosine10 (GAT) by mouse spleen cells in vitro.
Genetic control of immune responses in vitro. I. Development of primary and secondary plaque-forming cell responses to the random terpolymer 1-glutamic acid 60-1-alanine30-1-tyrosine10 (GAT) by mouse spleen cells in vitro.
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体外免疫反应的遗传控制。 I.在体外，小鼠脾细胞在体外开发了对随机晶状体1-谷氨酸60-1-丙氨酸30-1-丙氨酸30-1-丙氨酸10（GAT）的原发性和继发斑块形成细胞的反应。
DOI：
10.1084/jem.138.5.1107
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发表时间：
1973-11-01
期刊：
影响因子：
--
通讯作者：
Benacerraf B
中科院分区：
文献类型：
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作者：
Kapp JA;Pierce CW;Benacerraf B
在体内，小鼠对随机三元聚合物l -谷氨酸50- l -丙氨酸30- l -酪氨酸10 （GAT）的抗体反应是由组织相容性相关的免疫反应基因控制的。我们在体外研究了有应答小鼠和无应答小鼠脾脏细胞对GAT和GAT- mbsa复合物的抗体反应。使用GAT偶联绵羊红细胞作为指示细胞，在改进的Jerne斑块试验中列举了产生GAT特异性抗体的细胞。可溶性GAT刺激应答小鼠脾细胞、C57Bl/6 （H-2b）、F1 (C57 x SJL) （H-2b/s）和A/J （H-2a）培养中IgG GAT特异性斑块形成细胞（PFC）反应的发展。在无应答小鼠、SJL （H-2s）、B10的脾细胞培养中，可溶性GAT不会刺激GAT特异性PFC反应的产生。S （H-2s）和a.s.w （H-2s）。GAT-MBSA刺激应答株和非应答株小鼠脾细胞中IgG gat特异性PFC反应的发展。这些数据与通过血清学技术测量小鼠对GAT和GAT- mbsa有反应和无反应菌株的体内反应所获得的数据精确相关。因此，该体外系统可以有效地作为研究组织相容性相关免疫反应基因调控的细胞事件的模型。
In vivo, the antibody response in mice to the random terpolymer L-glutamic acid50-L-alanine30-L-tyrosine10 (GAT) is controlled by a histocompatibility-linked immune response gene(s). We have studied antibody responses by spleen cells from responder and nonresponder mice to GAT and GAT complexed to methylated bovine serum albumin (GAT-MBSA) in vitro. Cells producing antibodies specific for GAT were enumerated in a modified Jerne plaque assay using GAT coupled to sheep erythrocytes as indicator cells. Soluble GAT stimulated development of IgG GAT-specific plaque-forming cell (PFC) responses in cultures of spleen cells from responder mice, C57Bl/6 (H-2b), F1 (C57 x SJL) (H-2b/s), and A/J (H-2a). Soluble GAT did not stimulate development of GAT-specific PFC responses in cultures of spleen cells from nonresponder mice, SJL (H-2s), B10.S (H-2s), and A.SW (H-2s). GAT-MBSA stimulated development of IgG GAT-specific PFC responses in cultures of spleen cells from both responder and nonresponder strains of mice. These data correlate precisely with data obtained by measuring the in vivo responses of responder and nonresponder strains of mice to GAT and GAT-MBSA by serological techniques. Therefore, this in vitro system can effectively be used as a model to study the cellular events regulated by histocompatibility-linked immune response genes.