Ductal injection of preservation solution increases islet yields in islet isolation and improves islet graft function
Ductal injection of preservation solution increases islet yields in islet isolation and improves islet graft function
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DOI：
10.3727/000000008783907062
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发表时间：
2008-01-01
影响因子：
3.3
通讯作者：
Matsumoto, Shinichi
中科院分区：
文献类型：
--
作者：
Noguchi, Hirofumi;Ueda, Michiko;Matsumoto, Shinichi
对于胰岛移植，重要的是从单个供体胰腺获得足以逆转糖尿病的可用胰岛质量。最近的一份报告表明，使用M-Kyoto溶液代替UW溶液提高了双层胰腺保存方法中的胰岛产量。本研究调查了在胰腺储存前导管内注射大量保存液（UW和M-Kyoto溶液）是否能提高胰岛产量。导管内注射（+）组纯化前后的胰岛产量均显著高于导管内注射（-）组。与导管注射（-）组相比，导管注射（+）组中的TUNEL阳性细胞显著减少。在消化步骤中，胰管注射保存液增加了胰腺组织中的ATP水平并降低了胰蛋白酶活性。Annexin V和PI检测显示导管内注射可防止胰岛细胞凋亡。在移植模型中，导管注射改善了胰岛移植物的功能。这些结果表明，导管内注射保存液，特别是M-Kyoto溶液，可改善胰岛移植的结局。基于这些数据，该技术现在用于临床从无心跳供体胰腺或活体供体胰腺进行胰岛移植。
For islet transplantation, it is important to obtain an available islet mass adequate for diabetes reversal from a single donor pancreas. A recent report demonstrated that the use of M-Kyoto solution instead of UW solution improved islet yields in the two-layer method for pancreas preservation. The present study investigated whether the ductal injection of a large volume of preservation solution (UW and M-Kyoto solution) before pancreas storage improves islet yields. Islet yield both before and after purification was significantly higher in the ductal injection (+) group compared with the ductal injection (-) group. TUNEL-positive cells in the ductal injection (+) group were significantly decreased in comparison to the ductal injection (-) group. The ductal injection of preservation solution increased the ATP level in the pancreas tissue and reduced trypsin activity during the digestion step. Annexin V and PI assays showed that the ductal injection prevents islet apoptosis. In a transplant model, the ductal injection improved islet graft function. These findings suggest that the ductal injection of preservation solution, especially the M-Kyoto solution, leads to improved outcomes for pancreatic islet transplantation. Based on these data, this technique is now used for clinical islet transplantation from non-heart-beating donor pancreata or living donor pancreas.