Antagonistic activities of epiphytic bacteria from soybean leaves against Pseudomonas syringae pv glycinea in vitro and in planta
Antagonistic activities of epiphytic bacteria from soybean leaves against Pseudomonas syringae pv glycinea in vitro and in planta
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DOI：
10.1007/s002489900041
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发表时间：
1997-09-01
影响因子：
3.6
通讯作者：
Kampmann, G
中科院分区：
文献类型：
--
作者：
May, R;Volksch, B;Kampmann, G
在两个生长季节中，从大豆叶片中分离到273个细菌菌株，这些菌株没有和具有由假单胞菌引起的细菌性枯萎病症状。糖化血红蛋白（Psg）。从症状叶片分离的大多数菌株被鉴定为Psg（43%），其次是Erwinia dumicola（21%）和Enterobacter/Erwinia（19%）。从无症状叶片上分离到的菌株主要为一组未鉴定的革兰氏阴性菌（22%）、Psg（21%）和E.（18%）。Psg在腐殖菌之前定殖在大豆叶片上，并且在两个季节中在健康和感染的叶片上都保持优势。采用琼脂扩散法测定了82株Saplitic分离物对Psg的体外拮抗活性。对于植物体内测定，将Psg和每种分离物同时接种到温室生长的大豆植物的针刺叶的伤口中。29个菌株在体外具有拮抗作用。当菌株与病原菌混合接种时，19个菌株能够抑制Psg的生长并防止植物叶斑的形成。82个菌株中只有9个菌株在体外和植物体内都能抑制Psg。检测到的大多数拮抗菌属于假单胞菌属和欧文氏菌属。在植物测定应是一个可靠的预测田间性能的筛选生物防治剂。
Over two growing seasons, 273 bacterial strains were isolated from soybean leaves without and with bacterial blight symptoms caused by Pseudomonas syringae pv. glycinea (Psg). The majority of the isolates from leaves with symptoms were identified as Psg (43%), followed by Erwinia herbicola (21%), and Enterobacter/Erwinia (19%). The isolates from leaves without symptoms included mainly a group of unidentified Gram-negative bacteria (22%), Psg (21%), and E. herbicola (18%). Psg colonized the soybean leaves prior to saprophytic bacteria, and remained dominant during both seasons on healthy, as well as infected, leaves. Eighty-two saprophytic isolates were tested in vitro for their antagonistic activities against Psg, using an agar-diffusion assay. For the in planta assay, Psg and each isolate were simultaneously inoculated into wounds of pin-pricked leaves of greenhouse-grown soybean plants. Twenty-nine isolates were antagonistic in vitro. Nineteen isolates were able to suppress the growth of Psg and prevented the formation of leaf spots in planta when mixtures of isolate and pathogen were inoculated at ratios > 1. Only 9 of the 82 isolates inhibited Psg in vitro as well as in planta. Most antagonists detected belonged to the genera Pseudomonas and the species Erwinia herbicola. The in planta assay should be a reliable predictor of field performance for screening of biological control agents.