Regulation of ciliary polarity by the APC/C
Regulation of ciliary polarity by the APC/C
复制标题
DOI：
10.1073/pnas.0909465106
复制
发表时间：
2009-10-20
影响因子：
11.1
通讯作者：
Walz, Gerd
中科院分区：
文献类型：
--
作者：
Ganner, Athina;Lienkamp, Soeren;Walz, Gerd
平面细胞极性信号控制着多种极化细胞行为。在多毛爪蛇表皮细胞中，dishevelated （Dvl）向基底募集并定位到纤毛根的中心是运动纤毛正确定位的必要条件。我们现在报道，后期促进复合体（APC/C）识别Dvl的D-box基序，并在高度保守的赖氨酸残基上泛素化Dvl。通过敲低ANAPC2亚基抑制APC/C功能，破坏了运动纤毛的极性，改变了沿爪蟾胚胎表皮的流体运动的方向性。我们的研究结果表明，APC/C活性使爪蟾表皮细胞纤毛正确极化。
Planar cell polarity signaling controls a variety of polarized cell behaviors. In multiciliated Xenopus epidermal cells, recruitment of Dishevelled (Dvl) to the basal body and its localization to the center of the ciliary rootlet are required to correctly position the motile cilia. We now report that the anaphase-promoting complex (APC/C) recognizes a D-box motif of Dvl and ubiquitylates Dvl on a highly conserved lysine residue. Inhibition of APC/C function by knockdown of the ANAPC2 subunit disrupts the polarity of motile cilia and alters the directionality of the fluid movement along the epidermis of the Xenopus embryo. Our results suggest that the APC/C activity enables cilia to correctly polarize in Xenopus epidermal cells.