Genome-wide analysis and expression profiling of half-size ABC protein subgroup G in rice in response to abiotic stress and phytohormone treatments
Genome-wide analysis and expression profiling of half-size ABC protein subgroup G in rice in response to abiotic stress and phytohormone treatments
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DOI：
10.1007/s00438-012-0719-3
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发表时间：
2012-10-01
影响因子：
3.1
通讯作者：
Kato, Kiyoaki
中科院分区：
文献类型：
--
作者：
Matsuda, Shuichi;Funabiki, Atsushi;Kato, Kiyoaki
在双子叶模型拟南芥中，由半长的三磷酸腺苷结合盒转运体G亚组(ABCG)基因编码的蛋白质在非生物胁迫反应中的作用开始得到证实。在单子叶模式水稻中，大多数半大小的ABCG蛋白在非生物胁迫反应中的功能尚不清楚。Rcn1/OsABCG5是非生物胁迫下生长发育所必需的转运蛋白，但其分子功能仍不清楚。在这里，我们对水稻中所有30个半大小的ABCG基因进行了全面的综述，包括它们的基因结构、系统发育、染色体位置和保守基序。系统进化分析表明，半大小的OsABCG蛋白分为四类。所有7个水稻无内含子基因，包括Rcn1/OsABCG5，与拟南芥的12个无内含子ABCG基因一样，都属于III类基因。EST和FL-cDNA数据库提供了25个OsABCG基因的表达信息。半定量和定量RT-PCR分析表明，在非生物胁迫(6、17、42摄氏度、氯化钠或甘露醇)和脱落酸处理后，有7个OsABCG基因在幼苗、地上部或根中上调。除脱落酸外，另有15个OsABCG基因在至少一种非生物胁迫条件和其他植物激素条件下表达上调。基因表达谱的系统聚类分析表明，OsABCG基因的表达可分为四类。Rcn1/OsABCG5簇被脱落酸上调，包括OsABCG2、3、13和27。本研究将为进一步研究单子叶植物ABCGs的功能提供参考。
The roles of the proteins encoded by half-size adenosine triphosphate-binding cassette transporter subgroup G (ABCG) genes in abiotic stress responses are starting to be established in the dicot model Arabidopsis thaliana. In the monocot model rice, the functions of most half-size ABCG proteins in abiotic stress responses are unknown. Rcn1/OsABCG5 is an essential transporter for growth and development under abiotic stress, but its molecular function remains largely unclear. Here, we present a comprehensive overview of all 30 half-size ABCG genes in rice, including their gene structures, phylogeny, chromosome locations, and conserved motifs. Phylogenetic analysis revealed that the half-size OsABCG proteins were divided to four classes. All seven rice intronless genes, including Rcn1/OsABCG5, were in Class III, like the 12 intronless ABCG genes of Arabidopsis. The EST and FL-cDNA databases provided expression information for 25 OsABCG genes. Semi-quantitative and quantitative RT-PCR analyses demonstrated that seven OsABCG genes were up-regulated in seedlings, shoots or roots following treatments with abiotic stresses (6, 17, 42 A degrees C, NaCl, or mannitol) and abscisic acid. Another 15 OsABCG genes were up-regulated under at least one of the abiotic stress conditions and other phytohormones besides abscisic acid. Hierarchical clustering analysis of gene expression profiles showed that expression of the OsABCG genes could be classified into four clusters. The Rcn1/OsABCG5 cluster was up-regulated by abscisic acid and included OsABCG2, 3, 13, and 27. The present study will provide a useful reference for further functional analysis of the ABCGs in monocots.