Ultrasensitive and high specific detection of non-small-cell lung cancer cells in human serum and clinical pleural effusion by aptamer-based fluorescence spectroscopy
Ultrasensitive and high specific detection of non-small-cell lung cancer cells in human serum and clinical pleural effusion by aptamer-based fluorescence spectroscopy
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基于适配体的荧光光谱法超灵敏、高特异性检测人血清和临床胸腔积液中的非小细胞肺癌细胞
DOI：
10.1016/j.talanta.2017.11.029
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发表时间：
2018-03-01
期刊：
影响因子：
6.1
通讯作者：
Tang, Yalin
中科院分区：
文献类型：
--
作者：
Wu, Yuhang;Zhang, Hong;Tang, Yalin
肺癌是全球癌症相关死亡的主要原因，约85%被诊断为非小细胞肺癌（NSCLC）。然而，早期NSCLC的有效检测和诊断仍然具有挑战性。在这项工作中，我们开发了一种简单，超灵敏和高选择性的A549人NSCLC细胞检测策略，该策略基于结合新型花菁染料3，3 '-二氨基-N-甲基-N（3-磺丙基）-4，5，4 ′，5 ′-二苯并-9-甲基-硫代碳菁三乙基铵盐（cy-M）与对NSCLC细胞具有特异性亲和力的适体S6 G-四链体结构，其诱导显著的荧光增强（类似于10（4）倍）。此外，该方法还成功地应用于人血清和临床胸腔积液等复杂介质中A549癌细胞的检测，表明该方法可用于肺癌的早期诊断。
Lung cancer is the leading cause of cancer-related deaths worldwide, and approximately 85% are diagnosed as non-small-cell lung cancer (NSCLC). However, efficient detection and diagnosis of NSCLC at early stage is still challenging. In this work, we developed a simple, ultrasensitive and high selective strategy for A549 human NSCLC cells detection based on combining the reorganization property of a novel cyanine dye 3,3'-di(3-sulfopropyl)-4,5,4',5'-dibenzo-9-methyl-thiacarbocyanine triethylammonium salt (cy-M) to aptamer S6 G-quadruplex structure having specific affinity to NSCLC cells, which induced a dramatic fluorescence enhancement (similar to 10(4) times). Moreover, this strategy was successfully used for detecting of A549 cancer cells in complex media such as human serum and clinical pleural effusion, which strong indicated that the proposed method could be applied for the early diagnosis of lung cancer.