The Arabidopsis DELAYED DEHISCENCE1 gene encodes an enzyme in the jasmonic acid synthesis pathway
The Arabidopsis DELAYED DEHISCENCE1 gene encodes an enzyme in the jasmonic acid synthesis pathway
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DOI：
10.1105/tpc.12.7.1041
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发表时间：
2000-07-01
期刊：
影响因子：
11.6
通讯作者：
Goldberg, RB
中科院分区：
文献类型：
--
作者：
Sanders, PM;Lee, PY;Goldberg, RB
延迟开裂1是一种拟南芥T-DNA突变体，其中花药释放花粉粒太晚而不能进行授粉。延迟开裂1缺陷是由气孔退化程序延迟引起的。在延迟开裂1中被破坏的基因编码12-氧代植物二烯酸还原酶，这是茉莉酸生物合成途径中的酶。我们拯救了突变体表型外源应用茉莉酸，并获得种子集从以前的雄性不育植物。原位杂交研究表明，在花发育的早期阶段，延迟DEHISCE 1 mRNA积累在所有花器官。随后，延迟去HISCE 1 mRNA在雌蕊、花瓣和雄蕊花丝中特异性积累。延迟DEHISCE 1 mRNA在参与花粉释放的花药的气孔和隔细胞中未检测到。延迟开裂1中的T-DNA插入消除了延迟开裂1 mRNA积累和12-氧代植物二烯酸还原酶活性。这些实验表明，茉莉酸信号在控制花内花药开裂的时间中起作用。
delayed dehiscence1 is an Arabidopsis T-DNA mutant in which anthers release pollen grains too late for pollination to occur. The delayed dehiscence1 defect is caused by a delay in the stomium degeneration program. The gene disrupted in delayed dehiscence1 encodes 12-oxophytodienoate reductase, an enzyme in the jasmonic acid biosynthesis pathway. We rescued the mutant phenotype by exogenous application of jasmonic acid and obtained seed set from previously male-sterile plants. In situ hybridization studies showed that during the early stages of floral development, DELAYED DEHISCENCE1 mRNA accumulated within all floral organs. Later, DELAYED DEHISCENCE1 mRNA accumulated specifically within the pistil, petals, and stamen filaments. DELAYED DEHISCENCE1 mRNA was not detected in the stomium and septum cells of the anther that are involved in pollen release. The T-DNA insertion in delayed dehiscence1 eliminated both DELAYED DEHISCENCE1 mRNA accumulation and 12-oxophytodienoate reductase activity. These experiments suggest that jasmonic acid signaling plays a role in controlling the time of anther dehiscence within the flower.