The wavelength dependence of the response of a pulse fluorometer using the single photoelectron counting method
The wavelength dependence of the response of a pulse fluorometer using the single photoelectron counting method
复制标题
使用单光电子计数方法的脉冲荧光计响应的波长依赖性
DOI：
10.1063/1.1686442
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发表时间：
1974
影响因子：
1.6
通讯作者：
B. Donzel
中科院分区：
文献类型：
--
作者：
P. Wahl;J. Auchet;B. Donzel
我们研究了仪器响应函数g(T)，它是解卷积单光子计数法所获得的荧光衰变实验所必须知道的。由于闪光和PM的特性，g(T)取决于激发和发射波长。定性地分析了PM的贡献，发现其与光电效应的性质是一致的。因此，通常使用的方法是用PM接收激发脉冲的光所获得的仪器响应来代替g(T)，从而不能准确地测定“真正的荧光衰减”。函数g(T)可以通过使用具有已知荧光衰减时间的参考荧光溶液来获得。
We study the apparatus response function g (t) which must be known in order to deconvolute the fluorescence decay experiments obtained by the single photon counting method. Due to the flash and the PM characteristics, g (t) depends on the excitation and emission wavelengths. The PM contribution is analyzed qualitatively and found to be in agreement with the properties of the photoelectric effect. Therefore, the accurate determination of the ``true fluorescence decay'' cannot be obtained by the process commonly used, which consists in replacing g (t) by the apparatus response obtained with the PM receiving the light of the exciting pulse. The function g (t) may be obtained in using a reference fluorescent solution with a known fluorescence decay‐time.