NEW COLORIMETRIC MICRODETERMINATION OF FREE FATTY-ACIDS IN SERUM
NEW COLORIMETRIC MICRODETERMINATION OF FREE FATTY-ACIDS IN SERUM
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DOI：
10.1016/0009-8981(73)90468-3
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发表时间：
1973-01-01
影响因子：
5
通讯作者：
KITA, M
中科院分区：
文献类型：
--
作者：
NOMA, A;OKABE, H;KITA, M
基于游离脂肪酸-铜皂的形成，建立了测定100 μl血清中游离脂肪酸（FFA）的比色显微法。用氯仿-庚烷-甲醇提取游离脂肪酸。该溶剂体系几乎不提取磷脂。采用2-（2-噻唑偶氮）对甲酚比色法测定铜。这些改进使该方法比其他可用的比色法更敏感和稳定。所得结果与其他比色法的结果基本一致。
A colorimetric micromethod, based on the formation of FFA-Cu soaps, was described for the determination of free fatty acids (FFA) in 100 μl of serum. FFA was extracted with chloroform-heptane-methanol. Phospholipids were scarcely extracted by this solvent system. 2-(2-thiozolylazo)-p-cresol was chosen for the colorimetric determination of copper. These modifications rendered the method more sensitive and stable than other available colorimetric methods. Results obtained with the proposed method were in reasonable agreement with those obtained with other colorimetric methods.