Primary structure of the L subunit of the reaction center from Rhodopseudomonas sphaeroides.
Primary structure of the L subunit of the reaction center from Rhodopseudomonas sphaeroides.
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球形红假单胞菌反应中心 L 亚基的一级结构。
DOI：
10.1073/pnas.81.23.7303
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发表时间：
1984
影响因子：
11.1
通讯作者：
Simon,MI
中科院分区：
文献类型：
--
作者：
Williams,JC;Steiner,LA;Feher,G;Simon,MI
反应中心是一个完整的膜蛋白，与几个辅因子一起，介导细菌光合作用中的主要光化学事件。反应中心蛋白的三个亚基L、M和H的氨基末端序列和编码M亚基的结构基因的序列先前已被报道。在本研究中，我们发现编码L亚基的结构基因的3'端与编码M亚基的基因的5'端重叠8个碱基。L亚基的一级结构已从基因的核苷酸序列和蛋白质的氨基和羧基末端的分析中确定。许多胰蛋白酶和胰凝乳蛋白酶肽的序列用于证实核苷酸序列。L亚基由281个氨基酸组成（Mr 31，319），含有5个疏水片段。它与M亚基和被称为QB或Mr 32，000蛋白的植物类囊体蛋白同源。
The reaction center is an integral membrane protein that, together with several cofactors, mediates the primary photochemical events in bacterial photosynthesis. The amino-terminal sequences of the three subunits, L, M, and H, of the reaction center protein and the sequence of the structural gene encoding the M subunit have been reported previously. In the present study, we found that the 3' end of the structural gene encoding the L subunit overlaps by eight bases the 5' end of the gene encoding the M subunit. The primary structure of the L subunit has been determined from the nucleotide sequence of the gene and from analyses of the amino and carboxyl termini of the protein. The sequences of a number of tryptic and chymotryptic peptides were used to corroborate the nucleotide sequence. The L subunit was found to be composed of 281 amino acids (Mr 31,319) and to contain five hydrophobic segments. It is homologous to the M subunit and to a plant thylakoid protein referred to as the QB or Mr 32,000 protein.