Cationic Silica Nanoparticles are Efficiently Transferred into Mammalian Cells
Cationic Silica Nanoparticles are Efficiently Transferred into Mammalian Cells
复制标题
DOI：
10.1109/mhs.2007.4420867
复制
发表时间：
2007-12
期刊：
影响因子：
--
通讯作者：
Li Liu;T. Takenaka;A.A. Zinchenlco;N. Chen;S. Inagaki;H. Asada;T. Kishida;O. Mazda;S. Murata;K. Yoshikawa
中科院分区：
文献类型：
--
作者：
Li Liu;T. Takenaka;A.A. Zinchenlco;N. Chen;S. Inagaki;H. Asada;T. Kishida;O. Mazda;S. Murata;K. Yoshikawa
纳米颗粒作为非病毒载体，有望成为高效非病毒基因载体的候选者。我们制备了有机修饰的阳离子二氧化硅纳米粒子作为高性能非病毒载体。值得注意的是，DNA 和纳米颗粒的复合物被视为染色质的简单模型。此外，据报道，DNA的转录活性在与小纳米颗粒复合后得以保留，其中复合物形成的方式在体外强烈依赖于纳米颗粒的尺寸。另一方面，已证实在多胺和相关聚阳离子诱导的巨型DNA的致密小球状态下转录被完全抑制。我们正在进行的研究的未来目标是弄清楚纳米粒子是否可以用于调节基因组DNA的高阶结构，从而激活或抑制转录。在本研究中，我们进行了将纳米粒子转染到细胞中的实验。荧光共聚焦显微镜观察表明，纳米颗粒被有效地吸收到细胞的细胞质和细胞核中。重要的是，还证明纳米颗粒对细胞无毒。还观察到了细胞摄取纳米粒子的情况的差异。结果表明，有机改性的阳离子二氧化硅纳米粒子可能是下一类新型 DNA 结合剂，并在未来用于生物和生物医学应用。
Nanoparticles, as nonviral vectors, are expected as a candidate on highly effective non-viral gene carrier. We prepared organically modified cationic silica nanoparticles toward the high performance nonviral vector. It is to be noted that the complexes of DNA and nanoparticles are regarded as a simple model of chromatin. Furthermore, it is reported that the transcription activity of DNA is preserved after complexing with small nanoparticles, where the manner of complex formation is strongly dependent on the size of the nanoparticles in vitro. On the other hand, it is confirmed that transcription is completely inhibited in the compact globule state of giant DNA induced by polyamines and related polycations. The future goal of our undergoing studies is to make clear whether nanoparticles can be used for regulation of the higher-order structure of genomic DNA, and hence activate or inhibit transcription. In the present study, we performed experiments of transfecting nanoparticles into cell. Observation by fluorescence confocal microscopy shows that nanoparticles are effectively taken up in both cytoplasm and nucleus in cells. Importantly, it is also demonstrated that the nanoparticles are non-toxic for cell. The difference in the scenario of nanoparticles uptake by cells has been also observed. The results suggest that organically modified cationic silica nanoparticles may be the next new class of DNA binders and carries for biological and biomedical applications in the future.