Prevalence of HPV and Variation of HPV 16/HPV 18 E6/E7 Genes in Cervical Cancer in Women in South West China
Prevalence of HPV and Variation of HPV 16/HPV 18 E6/E7 Genes in Cervical Cancer in Women in South West China
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DOI：
10.1002/jmv.24043
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发表时间：
2014-11-01
影响因子：
12.7
通讯作者：
Sun, Qiangming
中科院分区：
文献类型：
--
作者：
Yang, Lijuan;Yang, Hongying;Sun, Qiangming
高危型HPV E6/E7的遗传变异可能与特定地理区域宫颈癌的发生有关。关于西南地区宫颈癌患者HPV感染率及E6/E7变异的研究报道较少。本研究旨在调查中国西南地区宫颈癌患者中HPV及其E6/E7变异的流行情况。基因分型后，对大多数流行的HR HPV样本的E6/E7基因进行测序和分析。然后构建系统发育树，其次是二级结构的多样性和选择压力的分析。HPV 16型（73.8%）和HPV 18型（16.4%）是宫颈癌患者中最常见的感染类型，其次是HPV 58型、HPV 56型和HPV 59型，与该地区门诊患者HPV 58型感染率较高不同。在HPV 16 E6中观察到18个单核苷酸变化，其中13/18个非同义突变（5个在β折叠中，2个在α螺旋中）。在HPV 16 E7中发现了10个单核苷酸变化，其中3/10为非同义突变。在HPV 18 E6中观察到三个单核苷酸变化，其中一个非同义突变，在HPV 18 E7中仅鉴定出一个同义突变。HPV 16 E6-D25 E、E7-N29 S和E7-T846 C（S95 S）表现出普遍的连锁突变。系统发育树显示，欧洲和亚洲血统是主要的模式。本研究有助于了解HR HPV的内在地理相关性和致癌潜力，并有助于进一步研究诊断，治疗和治疗性疫苗策略。J. Med. Virol. 86：1926-1936，2014. (c)2014年威利期刊公司
Genetic variations of High-Risk HPV E6/E7 may be associated with the development of cervical cancer in specific geographic regions. Few data have been reported about the HPV prevalence and E6/E7 variants among cervical cancer patients in Southwest China. This study was designed to investigate the prevalence of HPV and E6/E7 variants of most prevalent HPV among cervical cancer patients in Southwest China. After genotyping, E6/E7 genes of most prevalent HR HPV samples were sequenced and analyzed. Phylogenetic trees were then constructed, followed by an analysis of the diversity of secondary structure and selection pressures. HPV 16 (73.8%) and HPV 18 (16.4%) are the most prevalent infection types among cervical cancer patients, followed by HPV 58, HPV 56 and HPV 59, which is different from the high HPV 58 infection rate of outpatients in this region. Eighteen single nucleotide changes were observed in HPV 16 E6 with 13/18 non-synonymous mutations (5 in beta sheet and 2 in alpha helix). Ten single nucleotide changes were identified among HPV 16 E7 with 3/10 non-synonymous mutations. Three single nucleotide changes were observed in HPV 18 E6 with one non-synonymous mutation, and only one synonymous mutation was identified in HPV 18 E7. HPV 16 E6-D25E, E7-N29S and E7-T846C (S95S) exhibited a prevalent linkage mutation. The phylogenetic tree demonstrates that European and Asian lineages were the main patterns. This study may help understand the intrinsic geographical relatedness and oncogenic potential of HR HPV and contributes further to research of diagnostic, therapeutic and therapeutic vaccine strategy. J. Med. Virol. 86:1926-1936, 2014. (c) 2014 Wiley Periodicals, Inc.