Oxic and anoxic conditions affect arsenic (As) accumulation and arsenite transporter expression in rice.
Oxic and anoxic conditions affect arsenic (As) accumulation and arsenite transporter expression in rice.
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DOI：
10.1016/j.chemosphere.2016.10.114
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发表时间：
2017-02
期刊：
影响因子：
8.8
通讯作者：
Chuan Wu;Liu Huang;Sheng-guo Xue;Wei-song Pan;Qi Zou;W. Hartley;M. Wong
中科院分区：
文献类型：
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作者：
Chuan Wu;Liu Huang;Sheng-guo Xue;Wei-song Pan;Qi Zou;W. Hartley;M. Wong
食用大米引起的砷暴露现在已经成为一个全球健康问题。本研究旨在研究水稻根际氧化条件对水稻硅酸盐转运体(负责砷的运输)表达的影响，以及对两个杂交水稻品种(湘丰优9、沈优9586)和两个水稻亚种(湘晚稻17、湘晚稻12)砷积累和形态的影响。缺氧和缺氧处理对根长(p<0.001)和重量(p<0.05)有不同的影响。根中总砷浓度(88.8-218万mg/kg)显著低于缺氧处理(147243m g/kg)(p<0.001)。此外，根和地上部的亚砷酸盐浓度在亚砷酸盐处理中均低于缺氧处理。与缺氧处理相比，缺氧处理中硅酸盐转运蛋白的相对丰度呈下降趋势，尤其是湘晚鲜17、湘晚鲜12的Lsi1表达差异显著(p<0.05)，湘丰优9、神油9586、湘晚鲜17的Lsi2表达差异显著(p<0.05)。但不同AS处理和不同基因型之间的转运蛋白表达差异不显著。这可能是水稻在有氧条件下比淹水条件下砷积累量低的一个可能原因，也可能是降低水稻中砷健康风险的潜在途径。
Arsenic (As) exposure from rice consumption has now become a global health issue. This study aimed to investigate the effects of rice rhizosphere oxic conditions on silicate transporter (responsible for arsenite transportation) expressions, and on As accumulation and speciation in four rice genotypes, including two hybrid genotypes (Xiangfengyou9, Shenyou9586) and two indica subspecies (Xiangwanxian17, Xiangwanxian12). Oxic and anoxic treatments have different effects on root length (p < 0.001) and weight (p < 0.05). Total As concentrations in roots were dramatically lower in oxic treatments (88.8–218 mg/kg), compared to anoxic treatments (147–243 mg/kg) (p < 0.001). Moreover, root and shoot arsenite concentrations in oxic treatments were lower than that in anoxic treatments in arsenite treatments. The relative abundance of silicate transporter expressions displayed a trend of down-regulation in oxic treatments compared to anoxic treatments, especially significantly different for Xiangwanxian17, Xiangwanxian12 in Lsi1 expressions (p < 0.05), Xiangfengyou9, Shenyou9586, Xiangwanxian17 in Lsi2 expressions (p < 0.05). However, there were no significant differences of transporter expressions in different As treatments and genotypes. It may be a possible reason for low As accumulation in rice growing aerobically compared to flooded condition and a potential route to reduce the health risk of As in rice.