Calcium receptor messenger ribonucleic acid levels in the parathyroid glands and kidney of vitamin D-deficient rats are not regulated by plasma calcium or 1,25-dihydroxyvitamin D3.
Calcium receptor messenger ribonucleic acid levels in the parathyroid glands and kidney of vitamin D-deficient rats are not regulated by plasma calcium or 1,25-dihydroxyvitamin D3.
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维生素 D 缺乏大鼠的甲状旁腺和肾脏中钙受体信使核糖核酸水平不受血浆钙或 1,25-二羟基维生素 D3 的调节。
DOI：
10.1210/endo.136.2.7835282
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发表时间：
1995
期刊：
影响因子：
4.8
通讯作者：
John Fox
中科院分区：
文献类型：
--
作者：
K. Rogers;Christine K. Dunn;R. L. Conklin;S. Hadfield;B. Petty;E M Brown;Steven C. Hebert;Edward F. Nemeth;John Fox
细胞外离子钙（[Ca 2 +]o）水平是PTH分泌的主要生理调节因子。已经从牛和人的甲状旁腺中克隆了编码介导这种反应的钙受体（CaR）蛋白的互补DNA。该蛋白是一种七跨膜的G蛋白偶联受体，与细胞内Ca 2+的动员有关，以响应[Ca 2 +]o的增加。最近，大鼠肾脏CaR已被克隆，并显示在氨基酸水平上与牛甲状旁腺CaR有92%的相同性。已在许多细胞类型中记录了多种G蛋白偶联受体的表达和/或功能的同源或异源调节。因此，我们确定了PTH合成和分泌的主要调节剂[Ca 2 +]o和1，25-二羟维生素D3 [1，25-（OH）2D 3]是否影响大鼠甲状旁腺和肾脏中CaR基因的表达。使用溶液杂交测定法定量来自个体动物的成对甲状旁腺和单个肾脏中的CaR信使RNA（mRNA）水平。在维生素D缺乏（-D）大鼠中，单独评估Ca 2+和1，25-（OH）2D 3对CaR基因表达的影响。通过补充不同钙量的-D饮食和使用渗透微型泵静脉输注氯化钙7天，产生了广泛的血浆Ca 2+水平（0.7-1.9 mM）。血浆Ca ~（2+）水平与甲状旁腺（r = -0.18）和肾脏（r = 0.25）稳态CaR mRNA水平无相关性。在另一组-D大鼠中，以25和275 ng/kg.day皮下输注1，25-（OH）2D 3 10-12天。调整膳食钙以维持某些组的正常钙血症。无论产生的血浆Ca 2+或1，25-（OH）2D 3水平如何，1，25-（OH）2D 3给药对甲状旁腺或肾脏中的CaR mRNA水平均无影响。总之，无论是甲状旁腺还是肾脏CaR mRNA水平调节血浆Ca 2+和1，25-（OH）2D 3水平在这里检查的实验模型。
The level of extracellular ionized calcium ([Ca2+]o) is the primary physiological regulator of PTH secretion. Complementary DNAs encoding the calcium receptor (CaR) protein that mediates this response have been cloned from bovine and human parathyroid glands. This protein is a seven-transmembrane, G-protein-coupled receptor linked to the mobilization of intracellular Ca2+ in response to increases in [Ca2+]o. More recently, a rat kidney CaR has been cloned and shown to be 92% identical at the amino acid level to the bovine parathyroid CaR. Homologous or heterologous regulation of the expression and/or function of a variety of G-protein-coupled receptors has been documented in numerous cell types. Therefore, we determined whether [Ca2+]o and 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3], major regulators of PTH synthesis and secretion, affect CaR gene expression in parathyroid gland and kidney in rats. CaR messenger RNA (mRNA) levels were quantified in pairs of parathyroid glands and single kidneys from individual animals using a solution hybridization assay. The effects of Ca2+ and 1,25-(OH)2D3 on CaR gene expression were assessed independently in vitamin D-deficient (-D) rats. A wide range of plasma Ca2+ levels (0.7-1.9 mM) was produced by supplementing -D diets with varying amounts of calcium and by infusing CaCl2 i.v. for 7 days using osmotic minipumps. There was no correlation between plasma Ca2+ levels and steady state CaR mRNA levels in parathyroid gland (r = -0.18) or kidney (r = 0.25). In another group of -D rats, 1,25-(OH)2D3 was infused sc at 25 and 275 ng/kg.day for 10-12 days. Dietary calcium was adjusted to maintain normocalcemia in some of the groups. No effect of 1,25-(OH)2D3 administration on CaR mRNA levels occurred in parathyroid glands or kidney regardless of the resultant plasma Ca2+ or 1,25-(OH)2D3 levels. In conclusion, neither parathyroid gland nor kidney CaR mRNA levels are regulated by plasma Ca2+ and 1,25-(OH)2D3 levels in the experimental models examined here.
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兔主动脉平滑肌细胞中的 α1-肾上腺素能受体 mRNA 水平受去甲肾上腺素调节。
DOI：
10.1073/pnas.87.16.6268
发表时间：
1990
影响因子：
11.1
作者：
IzzoJr,NJ;Seidman,CE;Collins,S;Colucci,WS
通讯作者：
Colucci,WS
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DOI：
10.1113/jphysiol.1993.sp019765
发表时间：
1993
期刊：
The Journal of physiology
影响因子：
--
作者：
Racke,FK;Nemeth,EF
通讯作者：
Nemeth,EF
甲状旁腺激素基因的 5-侧翼区域介导 1,25-(OH)2 维生素 D3 的负调节。
DOI：
--
发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Okazaki,T;Igarashi,T;Kronenberg,HM
通讯作者：
Kronenberg,HM
DOI：
10.1210/jcem.76.3.8445032
发表时间：
1993-03
期刊：
The Journal of clinical endocrinology and metabolism
影响因子：
--
作者：
S. Khosla;P. Ebeling;A. Firek;M. F. Burritt;P. Kao;H. Heath
通讯作者：
S. Khosla;P. Ebeling;A. Firek;M. F. Burritt;P. Kao;H. Heath
牛甲状旁腺细胞胞质钙稳态及其蛋白激酶 C 的调节。
DOI：
10.1113/jphysiol.1993.sp019764
发表时间：
1993
期刊：
The Journal of physiology
影响因子：
--
作者：
Racke,FK;Nemeth,EF
通讯作者：
Nemeth,EF