Construction of EGFP-tagged rBCG of E.tenella and distribution in chickens
Construction of EGFP-tagged rBCG of E.tenella and distribution in chickens
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DOI：
10.1007/s11427-009-0008-5
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发表时间：
2009-03-01
期刊：
影响因子：
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通讯作者：
Cai YaNan
中科院分区：
文献类型：
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作者：
Wang QiuYue;Li JianHua;Cai YaNan
鸡球虫病是一种严重危害养禽业的寄生虫病。本研究构建了增强型绿色荧光蛋白（EGFP）标记的重组卡介苗（rBCG）与球虫菱形抗原的融合蛋白，用于鸡体内rBCG的追踪。rBCG pMV 361-Rho/EGFP免疫鸡1次，诱导产生体液免疫应答。用激光共聚焦显微镜观察rBCG在肝、脾、肺、肾和盲肠中的定植情况。实时荧光定量RT-PCR结果显示，菱形蛋白在免疫后第7天表达量上升，第14天达到高峰，第28天消失。这些结果对鸡球虫病rBCG疫苗的研制具有重要意义。
Chicken coccidiosis is a major parasitic disease with substantial economic burden to the poultry industry. Enhanced green fluorescent protein (EGFP) tagged recombinant Bacille Calmette-Guerin (rBCG), as a fusion protein with coccidian rhomboid antigen was constructed to track rBCG in vivo in chickens in this study. Immunization of chickens with one dose of rBCG pMV361-Rho/EGFP induced humoral immune response. The colonization of rBCG in liver, spleen, lung, kidney and caecum was observed by laser confocal microscopy. Real-time quantitative RT-PCR showed a rise expression level of rhomboid protein on the 7th day and a peak on the 14th day and disappearance on the 28th day after immunization. These results have significant implications for the development of rBCG vaccines against avian coccidiosis.