Developing New SSR Markers from EST of Wheat
Developing New SSR Markers from EST of Wheat
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发表时间：
2005
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通讯作者：
C. Jun;R. Zheng
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作者：
C. Jun;R. Zheng
小麦 EST 序列的不断增加为新 SSR 标记提供了潜在的宝贵来源。本研究从国际小麦科EST合作组织（ITEC）数据库的10 380个EST中鉴定出444个SSR-EST，占EST总数的4.1%。其中，发现34个二核苷酸重复和347个三核苷酸重复，分别占SSR-EST总数的7.7%和78.0%。设计了 135 个 cSSR 引物，对 175 个选定的 SSR-EST 中的侧翼 SSR 进行测序，其中 82 个引物在小麦中有效。 82 个 cSSR 引物中的 32 个位于除 21 条小麦染色体中的 2D、4B 和 4D 之外的 18 条小麦染色体上。
The growing availability of EST sequences from wheat provides a potential valuable source of new SSR markers. In this study, 444 SSR-ESTs from 10 380 ESTs in the International Triticeae EST Cooperative (ITEC) database, representing 4.1% of the total number of ESTs were identified. Among them, 34 dinucleotide repeats and 347 trinucleotide repeats were found, accounting for 7.7% and 78.0% of the total SSR-ESTs respectively. 135 cSSR primers were designed to sequence flanking SSRs from 175 selected SSR-ESTs, of which 82 primers were efficient in wheat. 32 of the 82 cSSR primers were located on 18 wheat chromosomes except for 2D, 4B and 4D of 21 wheat chromosomes.