Diet-dependent, microbiota-independent regulation of IL-10-producing lamina propria macrophages in the small intestine.
Diet-dependent, microbiota-independent regulation of IL-10-producing lamina propria macrophages in the small intestine.
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DOI：
10.1038/srep27634
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发表时间：
2016-06-15
影响因子：
4.6
通讯作者：
Kamada N
中科院分区：
文献类型：
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作者：
Ochi T;Feng Y;Kitamoto S;Nagao-Kitamoto H;Kuffa P;Atarashi K;Honda K;Teitelbaum DH;Kamada N
肠道常驻巨噬细胞通过产生一种抗炎细胞因子白细胞介素-10来调节胃肠道稳态。虽然需要循环单核细胞的不断补充来维持结肠粘膜内的常驻ϕ池，但小肠（SI）中m φ的稳态调节尚不清楚。在这里，我们证明了膳食氨基酸的直接刺激可以调节SI中肠道内环境的稳态。给小鼠全肠外营养（TPN），剥夺动物的肠内营养，在SI中显示产生il -10的membros显著减少，而产生il -10的CD4+ T细胞保持完整。同样，肠内营养剥夺选择性地影响单核细胞来源的F4/80+ Mϕ群体，但不影响非单核细胞前体来源的CD103+树突状细胞。值得注意的是，与结肠型模态分布相反，SI模态分布的补充及其IL-10的产生不受肠道菌群的调节。相反，SI - md - s直接受到膳食氨基酸的调节。总的来说，我们的研究强调了SI中产生il -10的常驻模态的饮食依赖，微生物独立的调节。
Intestinal resident macrophages (Mϕs) regulate gastrointestinal homeostasis via production of an anti-inflammatory cytokine interleukin (IL)-10. Although a constant replenishment by circulating monocytes is required to maintain the pool of resident Mϕs in the colonic mucosa, the homeostatic regulation of Mϕ in the small intestine (SI) remains unclear. Here, we demonstrate that direct stimulation by dietary amino acids regulates the homeostasis of intestinal Mϕs in the SI. Mice that received total parenteral nutrition (TPN), which deprives the animals of enteral nutrients, displayed a significant decrease of IL-10-producing Mϕs in the SI, whereas the IL-10-producing CD4+ T cells remained intact. Likewise, enteral nutrient deprivation selectively affected the monocyte-derived F4/80+ Mϕ population, but not non-monocytic precursor-derived CD103+ dendritic cells. Notably, in contrast to colonic Mϕs, the replenishment of SI Mϕs and their IL-10 production were not regulated by the gut microbiota. Rather, SI Mϕs were directly regulated by dietary amino acids. Collectively, our study highlights the diet-dependent, microbiota-independent regulation of IL-10-producing resident Mϕs in the SI.