Perfluorinated alginate for cellular encapsulation
Perfluorinated alginate for cellular encapsulation
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DOI：
10.1002/jbm.a.34052
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发表时间：
2012-08-01
影响因子：
4.9
通讯作者：
Stabler, Cherie L.
中科院分区：
文献类型：
--
作者：
Gattas-Asfura, Kerim M.;Fraker, Christopher A.;Stabler, Cherie L.
利用线性聚乙二醇键和酰胺键将十五氟辛酰氯(PFC)分子接枝到海藻酸盐(Alg)上。用质子核磁共振和红外光谱对Alg-PFC材料进行了表征。PFC的功能化程度显著影响了Alg-PFC的物理和化学性质，特别是当所得到的聚合物被离子交联成水凝胶时。通过Ba2+交联制备的ALG-PFC水凝胶珠被发现与对照海藻酸盐珠的渗透性相匹配，除了在培养介质中随着时间的推移而膨胀。当用于包裹MIN6细胞时，Alg-PFC微球比藻酸盐对照微球显示出促进细胞增殖的作用。这些结果表明，Alg-PFC水凝胶保留了PFC的一些与生物相关的优点，如增强质量传输和生物惰性，以提高藻酸盐三维水凝胶环境中的细胞活力。我们预计这些功能化的水凝胶将在包裹具有高代谢需求的细胞方面特别有用，例如胰岛。(C)2012年威利期刊，Inc.生物材料研究公司2012年A部分。
Molecules of pentadecafluorooctanoyl chloride (PFC) were grafted onto alginate (Alg) using a linear poly(ethylene glycol) linker and amide bonds. The resulting Alg-PFC material was characterized by proton nuclear magnetic resonance and infrared spectroscopies. The degree of PFC functionalization significantly influenced the physical and chemical properties of Alg-PFC, particularly when the resulting polymer was ionically crosslinked into hydrogels. Alg-PFC hydrogel beads fabricated via Ba2+ crosslinking were found to match the permeability properties of control alginate beads, except upon swelling over time in culture media. When used to encapsulate MIN6 cells, a beta cell line, Alg-PFC beads demonstrated enhanced cell proliferation over alginate control beads. These results indicate that Alg-PFC hydrogels retain some of the PFC's biological-relevant benefits, such as enhancement of mass transport and bioinertness, to enhance cellular viability within alginate three-dimensional hydrogel environments. We envision these functionalized hydrogels to be particularly useful in the encapsulation of cells with a high metabolic demand, such as pancreatic islets. (C) 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A 2012.