Non-abelian cohomology and extensions of Lie algebras
Non-abelian cohomology and extensions of Lie algebras
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发表时间：
2006
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通讯作者：
N. Inassaridze;E. Khmaladze;M. Ladra
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作者：
N. Inassaridze;E. Khmaladze;M. Ladra
一种用于通过含有至少50%甲酰胺或其它变性有机溶剂的至少12%聚合的线性丙烯酰胺凝胶分离5'-硫代磷酸酯单核苷酸混合物、5'-硫代磷酸酯寡核苷酸混合物或包含电泳的5'-硫代磷酸酯单核苷酸和5'-硫代磷酸酯寡核苷酸混合物的方法。这种方法可以分辨只有一个碱基不同的硫代寡核苷酸。在微升体积小于1纳克的样品可以方便地处理。
A method for separating a mixture of 5'-phosphorothioate mononucleotides, a mixture of 5'-phosphorothioate oligonucleotides, or a mixture of both 5'-phosphorothioate mononucleotides and 5'-phosphorothioate oligonucleotides comprising electro- phoresis through a gel of at least 12% polymerized linear acrylamide containing at least 50% formamide or other denaturing organic solvent. This method can resolve phosphorothioate oligonucleotides differing by only a single base. Samples of less than 1 nanogram in microliter volumes can be conveniently handled.