Nine-month persistence of Bursaphelenchus xylophilus in living Abies sachalinensis and Picea jezoensis seedlings and nematode reproduction in the stem sections
Nine-month persistence of Bursaphelenchus xylophilus in living Abies sachalinensis and Picea jezoensis seedlings and nematode reproduction in the stem sections
复制标题
松材线虫在活体冷杉和油皮云杉幼苗中的九个月持久性以及茎部线虫的繁殖
作者：
Kasuga;H.
松材线虫在感病松树上引起松树枯萎病。以确定是否B。在冷杉（Abies sachalinensi）和云杉（Picea jezoensis）人工林中，分别用10株和15株冷杉（A. sachalinensis，P.jezoensis，Pinus densiflora和P.雷鸣般的。接种15000B。在A. sachalinensis和P. jezoensis幼苗，表明对A. sachalinenses和P. jezoensis。相比之下，B.木虱对杨树的致死率分别为40%和70%。densiflora和P.在接种后9.5个月，从前两个树种的外部无症状幼苗的茎中以极低的密度回收了松材线虫。B. xylophilus在A. sachalinensis和P. 3周后，jezoensis幼苗在树皮中的种群密度比木质部增加得更多，而在P.密花茎切片显示两种组织之间的线虫密度没有差异。结果表明，B. xylophilus可能在含有A. sachalinensis和P. jezoensis，当存在对线虫具有亲和力的昆虫载体时。
Bursaphelenchus xylophiluscauses pine wilt disease in susceptible pine species. To determine whetherB. xylophiluspersists in forests containingAbies sachalinensisandPicea jezoensis, we performed inoculation experiments using ten or 15 seedlings ofA. sachalinensis,P. jezoensis,Pinus densifloraandP. thunbergii. Inoculation of 15 000B. xylophiluscaused 20 and 7% mortality inA. sachalinensisandP. jezoensisseedlings, respectively, in the nursery, indicating low susceptibility ofA. sachalinensisand a lack of susceptibility ofP. jezoensis. By contrast,B. xylophiluscaused 40 and 70% mortality inP. densifloraandP. thunbergii, respectively.Bursaphelenchus xylophiluswas recovered at extremely low densities from stems of externally asymptomatic seedlings of the first two tree species 9.5 months after inoculation. Inoculation ofB. xylophiluson excised stem sections ofA. sachalinensisandP. jezoensisseedlings showed a greater increase in population density in the bark than xylem after 3 weeks, whereas that onP. densiflorastem sections showed no difference in nematode density between the two tissues. The results suggest thatB. xylophilusmay persist in forests containingA. sachalinensisandP. jezoensis, when there are insect vectors with affinity for the nematode.