Silver colloid nanoparticles:: Synthesis, characterization, and their antibacterial activity
Silver colloid nanoparticles:: Synthesis, characterization, and their antibacterial activity
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DOI：
10.1021/jp063826h
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发表时间：
2006-08-24
影响因子：
3.3
通讯作者：
Zboril, Radek
中科院分区：
文献类型：
--
作者：
Panacek, Ales;Kvitek, Libor;Zboril, Radek
提出了一种一步简单合成粒径可控的银胶体纳米粒子的方法。在该合成中，通过四个还原剂进行[Ag（NH3）（2）]（+）络合物阳离子的还原。使用了四种单糖：两种单糖（葡萄糖和半乳糖）和两种二糖（麦芽糖和乳糖）。在各种氨浓度（0.005-0.20 mol L-1）和pH条件（11.5-13.0）下进行的合成产生了宽范围的粒径（25-450 nm）和窄的粒径分布，特别是在最低氨浓度下。通过动态光散射（DLS）、透射电子显微镜（TEM）和紫外/可见吸收分光光度法测定颗粒的平均尺寸、尺寸分布、形态和结构。糖结构（单糖与二糖）对银粒子的大小的影响进行了简要讨论。麦芽糖还原[Ag（NH3）（2）]（+）产生的银颗粒具有窄的粒径分布，平均粒径为25 nm，其对革兰氏阳性和革兰氏阴性细菌（包括高度多重耐药菌株，如耐甲氧西林金黄色葡萄球菌）显示出高的抗菌和杀菌活性。发现银纳米颗粒的抗菌活性依赖于银颗粒的尺寸。非常低浓度的银（低至1.69 μ g/mL Ag）产生抗菌性能。
A one-step simple synthesis of silver colloid nanoparticles with controllable sizes is presented. In this synthesis, reduction of [Ag(NH3)(2)](+) complex cation by four saccharides was performed. Four saccharides were used: two monosaccharides (glucose and galactose) and two disaccharides (maltose and lactose). The syntheses performed at various ammonia concentrations (0.005-0.20 mol L-1) and pH conditions (11.5-13.0) produced a wide range of particle sizes (25-450 nm) with narrow size distributions, especially at the lowest ammonia concentrations. The average size, size distribution, morphology, and structure of particles were determined by dynamic light scattering (DLS), transmission electron microscopy (TEM), and UV/Visible absorption spectrophotometry. The influence of the saccharide structure (monosacharides versus disaccharides) on the size of silver particles is briefly discussed. The reduction of [Ag(NH3)(2)](+) by maltose produced silver particles with a narrow size distribution with an average size of 25 nm, which showed high antimicrobial and bactericidal activity against Gram-positive and Gram-negative bacteria, including highly multiresistant strains such as methicillin-resistant Staphylococcus aureus. Antibacterial activity of silver nanoparticles was found to be dependent on the size of silver particles. A very low concentration of silver (as low as 1.69 mu g/mL Ag) gave antibacterial performance.