Distinct target-derived signals organize formation, maturation, and maintenance of motor nerve terminals
Distinct target-derived signals organize formation, maturation, and maintenance of motor nerve terminals
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DOI：
10.1016/j.cell.2007.02.035
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发表时间：
2007-04-06
期刊：
影响因子：
64.5
通讯作者：
Umemori, Hisashi
中科院分区：
文献类型：
--
作者：
Fox, Michael A.;Sanes, Joshua R.;Umemori, Hisashi
靶源性因子通过促进突触部位神经末梢的分化来组织突触发生。一些候选的组织分子已被确定基于它们的体外生物活性，但很少有人知道它们在体内的作用。在这里，我们表明，三组组织者依次采取行动的模式运动神经末梢：FGF，β 2层粘连蛋白，和胶原蛋白α（IV）链。7/10/22亚家族的FGF和广泛分布的胶原IV链（α 1/2）促进突触囊泡的成簇，作为神经末梢形成。集中在突触部位的β 2层粘连蛋白对于神经末梢的胚胎发育是必需的，但对于它们的出生后成熟是必需的。突触特异性胶原IV链（α 3-6）仅在突触成熟后积累，并且是突触维持所需的。因此，多个靶源信号允许离散控制突触前特化的形成、成熟和维持。
Target-derived factors organize synaptogenesis by promoting differentiation of nerve terminals at synaptic sites. Several candidate organizing molecules have been identified based on their bioactivities in vitro, but little is known about their roles in vivo. Here, we show that three sets of organizers act sequentially to pattern motor nerve terminals: FGFs, beta 2 laminins, and collagen alpha(IV) chains. FGFs of the 7/10/22 subfamily and broadly distributed collagen IV chains (alpha 1/2) promote clustering of synaptic vesicles as nerve terminals form. beta 2 laminins concentrated at synaptic sites are dispensable for embryonic development of nerve terminals but are required for their postnatal maturation. Synapse-specific collagen IV chains (alpha 3-6) accumulate only after synapses are mature and are required for synaptic maintenance. Thus, multiple target-derived signals permit discrete control of the formation, maturation, and maintenance of presynaptic specializations.