Low-power XiX decoupling in MAS NMR experiments
Low-power XiX decoupling in MAS NMR experiments
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DOI：
10.1016/s1090-7807(03)00155-1
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发表时间：
2003-08-01
影响因子：
2.2
通讯作者：
Meier, BH
中科院分区：
文献类型：
--
作者：
Ernst, M;Samoson, A;Meier, BH
介绍了在快速魔角旋转下实现低功率XIX质子解耦的方法。该方法适用于MAS频率超过质子-质子相互作用的情况。对于刚性有机固体，这是MAS频率在大约40 kHz以上的情况。结果表明，与低功率连续波解耦相比，解耦的质量和对频偏的灵敏度都有所提高。与优化的高功率去偶联相比，去偶联效率略有降低：在以50 kHz的MAS频率研究的多肽样品中，观察到CH3和CH基团的信号强度损失约10%，CH基团的信号强度损失约40%。考虑到在低功率XIX中的RF幅度比在高功率XIX去耦合中的RF幅度低约15倍，这种线路强度的降低有时是可以容忍的。(C)2003年埃尔塞维尔科学公司(美国)。版权所有。
Low-power XiX proton decoupling under fast magic-angle spinning is introduced. The method is applicable if the MAS frequency exceeds the proton-proton interactions. For rigid organic solids this is the case for MAS frequencies above approximately 40 kHz. It is shown that the quality of the decoupling as well as the sensitivity to frequency offsets can be improved compared to low-power continuous-wave decoupling. The decoupling efficiency is somewhat reduced compared to optimized high-power decoupling: in a peptide sample investigated at an MAS frequency of 50 kHz a loss of about 10% in signal intensity for CH3 and CH groups, and of about 40% for CH, groups was observed. Taking into consideration, that the rf amplitude in the low-power XiX was about 15 times lower than in high-power XiX decoupling, such a reduction in line intensity is sometimes tolerable. (C) 2003 Elsevier Science (USA). All rights reserved.