Shaping plasticity: Alterations in glutamate transporter localization as a pathophysiological mechanism in severe mental illness
Shaping plasticity: Alterations in glutamate transporter localization as a pathophysiological mechanism in severe mental illness
复制标题
塑造可塑性：谷氨酸转运蛋白定位的改变作为严重精神疾病的病理生理机制
DOI：
10.1038/mp.2016.79
复制
发表时间：
2016
影响因子：
11
通讯作者：
J. Meador
中科院分区：
文献类型：
--
作者：
R. Mccullumsmith;S. O’Donovan;J. Drummond;F. Benesh;M. Simmons;R. Roberts;T. Lauriat;V. Haroutunian;J. Meador
精神分裂症中谷氨酸转运蛋白剪接变体表达从星形胶质细胞到神经元的转变。人丘脑中 EAAT2 (pan) mRNA 的原位杂交放射自显影图 (a)。突出显示的区域是丘脑的内侧核。背核 20 μm 组织切片 (b) 的尼氏染色，放大 20 倍。根据形态识别中继神经元（大细胞、大圆圈）和星形胶质细胞（小细胞、小圆圈）。使用激光捕获显微切割从精神分裂症受试者和对照受试者中收获大细胞和小细胞。使用双重免疫荧光证实了精神分裂症中继神经元中 EAAT2 蛋白的表达 (c)。白色箭头表示 NeuN 和 EAAT2（黄色）双重标记的细胞。对从星形胶质细胞（小细胞）和中继神经元（大细胞）富集群中分离出的兴奋性氨基酸转运蛋白 EAAT2B 转录物进行 QPCR 分析 (d)。有关此主题的更多信息，请参阅 McCullumsmith 等人的文章。第 823–830 页。
A shift in glutamate transporter splice variant expression from astrocytes to neurons in schizophrenia. Autoradiogram (a) of in situ hybridization for EAAT2 (pan) mRNA in the human thalamus. The highlighted region is the mediodorsal nucleus of the thalamus. Nissl stain of a 20-μm tissue section (b) from the mediodorsal nucleus, x20 magnification. Relay neurons (large cells, big circles) and astrocytes (small cells, small circles) were identified based on morphology. Large and small cells were harvested from subjects with schizophrenia and control subjects using laser-capture microdissection. Expression of EAAT2 protein in relay neurons in schizophrenia was confirmed using dual immunofluorescence (c). The white arrows indicate cells double labeled for NeuN and EAAT2 (yellow). QPCR analysis (d) of excitatory amino-acid transporter EAAT2B transcripts isolated from enriched populations of astrocytes (small cells) and relay neurons (large cells). For more information on this topic, please refer to the article by McCullumsmith et al. on pages 823–830.