Quantitative nuclear magnetic resonance spectroscopic determination of the oxyethylene group content of polysorbates
Quantitative nuclear magnetic resonance spectroscopic determination of the oxyethylene group content of polysorbates
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核磁共振波谱定量测定聚山梨酯中氧乙烯基含量
DOI：
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发表时间：
2007
期刊：
影响因子：
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通讯作者：
K. Tanamoto
中科院分区：
文献类型：
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作者：
N. Sugimoto;R. Koike;Noriko Furusho;M. Tanno;C. Yomota;Kyoko Sato;T. Yamazaki;K. Tanamoto
聚山梨醇酯的环氧乙烷（EO）含量指南由联合国粮食及农业组织/世界卫生组织食品添加剂联合专家委员会制定。然而，经典的滴定法测定EO是困难和耗时的。在这里，我们表明，定量1H-核磁共振光谱可以快速，简单地确定聚山梨醇酯的EO含量。通过与脱水山梨糖醇单月桂酸酯和聚（乙二醇）二硬脂酸酯的比较，识别EO信号。邻苯二甲酸氢钾用作内标。根据EO与内标物的信号强度比值估计EO含量。两个核磁共振系统被用来验证所提出的方法。使用该技术测定市售聚山梨醇酯20、60、65和80的EO含量在推荐限度内。因此，我们的方法代表了测定聚山梨醇酯EO含量的附加或替代方法。
Guidelines for the oxyethylene group (EO) content of polysorbates are set by the Food and Agriculture Organization/World Health Organization Joint Expert Committee on Food Additives. However, the classical titration method for EO determination is difficult and time-consuming. Here, we show that quantitative 1H-nuclear magnetic resonance spectroscopy can determine the EO contents of polysorbates rapidly and simply. The EO signals were identified through comparisons with sorbitan monolaurate and poly(ethylene glycol) distearate. Potassium hydrogen phthalate was used as an internal standard. The EO contents were estimated from the ratio of the signal intensities of EO to the internal standard. Two nuclear magnetic resonance systems were used to validate the proposed method. The EO content of commercial polysorbates 20, 60, 65, and 80 was determined to be within the recommended limits using this technique. Our approach thus represents an additional or alternative method of determining the EO contents of polysorbates.