Perception of root-active CLE peptides requires CORYNE function in the phloem vasculature.
Perception of root-active CLE peptides requires CORYNE function in the phloem vasculature.
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DOI：
10.15252/embr.201643535
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发表时间：
2017-08
期刊：
影响因子：
7.7
通讯作者：
Hardtke CS
中科院分区：
文献类型：
--
作者：
Hazak O;Brandt B;Cattaneo P;Santiago J;Rodriguez-Villalon A;Hothorn M;Hardtke CS
拟南芥根的发育是由不同的CLAVATA 3/胚周围区（CLE）肽配体及其同源CLAVATA（CLV）和几乎任何分生组织（BAM）受体组成的信号传导途径协调的。不同CLE肽如何以及在何处触发根中的特定形态学或生理学变化知之甚少。在这里，我们报告说，受体样蛋白CLAVATA 2（CLV 2）和假激酶CORYNE（CRN）是完全感知根活性CLE肽所必需的。我们发现BAM 3作为CLE 45受体在根和生化地图其肽结合表面。与其他植物肽受体相反，我们没有发现体细胞胚胎发生受体激酶（SERK）蛋白在CLE 45感知中作为共受体激酶的证据。CRN稳定BAM 3表达，因此是BAM 3介导的CLE 45信号传导所必需的。此外，原生韧皮部特异性CRN表达补充了crn突变体对根活性CLE肽的抗性，表明原生韧皮部是它们的主要作用位点。我们的工作定义了一个遗传框架解剖CLE肽信号和CLV/BAM受体激活的根。
Arabidopsis root development is orchestrated by signaling pathways that consist of different CLAVATA3/EMBRYO SURROUNDING REGION (CLE) peptide ligands and their cognate CLAVATA (CLV) and BARELY ANY MERISTEM (BAM) receptors. How and where different CLE peptides trigger specific morphological or physiological changes in the root is poorly understood. Here, we report that the receptor‐like protein CLAVATA 2 (CLV2) and the pseudokinase CORYNE (CRN) are necessary to fully sense root‐active CLE peptides. We uncover BAM3 as the CLE45 receptor in the root and biochemically map its peptide binding surface. In contrast to other plant peptide receptors, we found no evidence that SOMATIC EMBRYOGENESIS RECEPTOR KINASE (SERK) proteins act as co‐receptor kinases in CLE45 perception. CRN stabilizes BAM3 expression and thus is required for BAM3‐mediated CLE45 signaling. Moreover, protophloem‐specific CRN expression complements resistance of the crn mutant to root‐active CLE peptides, suggesting that protophloem is their principal site of action. Our work defines a genetic framework for dissecting CLE peptide signaling and CLV/BAM receptor activation in the root.