Baculovirus-mediated promoter assay and transcriptional analysis of white spot syndrome virus orf427 gene.
Baculovirus-mediated promoter assay and transcriptional analysis of white spot syndrome virus orf427 gene.
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DOI：
10.1186/1743-422x-2-71
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发表时间：
2005-08-23
期刊：
影响因子：
4.8
通讯作者：
Kwang J
中科院分区：
文献类型：
--
作者：
Lu L;Wang H;Manopo I;Yu L;Kwang J
白色斑点综合征病毒（White Spot Syndrome Virus，WSSV）是对虾的一种重要病原，具有较高的致死率。WSSV的Orf 427基因是WSSV的三个主要潜伏相关基因之一。在此，我们有兴趣分析orf 427的启动子及其在病毒致病过程中的表达。原位杂交结果表明，ORF 427在病毒裂解感染过程中在所有感染组织中均有转录，并且可以从感染的虾中检测到翻译产物。时程RT-PCR分析表明，orf 427的转录产物只能检测到后6小时病毒接种。此外，杆状病毒介导的启动子分析表明，orf 427的启动子不能在昆虫SF 9细胞和原代虾细胞中表达EGFP报告基因。我们的数据表明，潜伏期相关的orf 427可能没有发挥重要作用，激活病毒复制从潜伏期，由于其后期转录裂解感染。
White spot syndrome virus (WSSV) is an important pathogen of the penaeid shrimp with high mortalities. In previous reports, Orf427 of WSSV is characterized as one of the three major latency-associated genes of WSSV. Here, we were interested to analyze the promoter of orf427 and its expression during viral pathogenesis. in situ hybridization revealed that orf427 was transcribed in all the infected tissues during viral lytic infection and the translational product can be detected from the infected shrimp. A time-course RT-PCR analysis indicated that transcriptional products of orf427 could only be detected after 6 h post virus inoculation. Furthermore, a baculovirus-mediated promoter analysis indicated that the promoter of orf427 failed to express the EGFP reporter gene in both insect SF9 cells and primary shrimp cells. Our data suggested that latency-related orf427 might not play an important role in activating virus replication from latent phase due to its late transcription during the lytic infection.