GPR30 is overexpressed in post-puberal testicular germ cell tumors
GPR30 is overexpressed in post-puberal testicular germ cell tumors
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DOI：
10.4161/cbt.11.6.14672
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发表时间：
2011-03-15
影响因子：
3.6
通讯作者：
Chieffi, Paolo
中科院分区：
文献类型：
--
作者：
Franco, Renato;Boscia, Francesca;Chieffi, Paolo
GPR 30是一种7跨膜G蛋白偶联的雌激素受体，与传统的雌激素受体一起调节细胞对17 β-雌二醇和环境雌激素的反应。GPR 30蛋白在所有小管内生殖细胞肿瘤、乳腺癌和胚胎性癌中均呈高水平表达，而在畸胎瘤中表达较低。免疫组化结果进一步证实了GPR 30蛋白的表达。我们还分析了GPR 30蛋白在来源于小鼠永生化B型精原细胞的GC 1和来源于人乳腺癌的TCam-2细胞中的表达。特异性GPR 30抑制剂的设计可能是一个有用的分子靶点，以阻断具有高增殖率的肿瘤生殖细胞，用于治疗TGCT。
GPR30 is a 7-transmembrane G protein-coupled estrogen receptor that functions alongside traditional estrogen receptors to regulate cellular responses to 17 beta-estradiol and environmental estrogens.In this study, we have evaluated by immunohistochemical analysis GPR30 expression in post-puberal testicular germ cell tumors (30 seminomas, 5 teratomas, 12 embryonal carcinomas and 20 intratubular germ cell tumors). The GPR30 protein expression was detected at high level in all intratubular germ cell tumors, seminomas and embryonal carcinomas, whereas in teratomas the expression was low. The immunohistochemical data were further confirmed by western blot analysis.GPR30 protein expression has also been analyzed in GC1 and TCam-2 cell lines, respectively derived from immortalized type B murine spermatogonia and human seminoma.Our results indicate that GPR30 could be a potential therapeutic target; the design of a specific GPR30 inhibitors could be a useful molecular target to block neoplastic germ cells with a high proliferative rate for the treatment of TGCTs.