Age-dependent anatomical changes in an identified neuron in the CNS of Aplysia californica.
Age-dependent anatomical changes in an identified neuron in the CNS of Aplysia californica.
复制标题
加州海兔中枢神经系统中已识别神经元的年龄依赖性解剖学变化。
DOI：
10.1002/neu.480120505
复制
发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Becker,J
中科院分区：
文献类型：
--
作者：
Papka,R;Peretz,B;Tudor,J;Becker,J
Aaplasia californica的神经元是天然着色的，并且色素随着年龄的增长而积累。在本研究中，色素检查在同一神经元从Aaplasia的三个postmetamorphic年龄：年轻，性成熟，和老年。通过光学和电子显微镜检查大型中央神经元R2，以确定该色素是否具有与衰老哺乳动物神经元中所见的脂褐素色素相似的性质。我们使用相同的显微镜技术，证明哺乳动物神经元中存在脂褐素。光学显微镜研究表明，自发荧光，染色与苏丹黑，和自然发生的色素在老R2之间的区域相关性。电子显微镜研究显示，在老年R2的外周细胞质中存在大的空泡化和层状膜结合体，与哺乳动物神经元中发现的相似。这些尸体位于观察到自发荧光和苏丹黑染色的同一区域。虽然天然存在的色素随着年龄的增长而积累，但它在老年性成熟动物的神经元中获得脂褐素色素的特征。这些色素特征的存在可以作为老化的指标，在神经元，因为它们是在哺乳动物的神经元。
Neurons ofAplysia californicaare naturally pigmented and the pigment accumulates with age. In the present study the pigment was examined in the same neuron fromAplysiaof three postmetamorphic ages: young, sexually mature, and old. The large central neuron, R2, was examined by light and electron microscopy to determine if the pigment possessed properties similar to lipofuscin pigment seen in aging mammalian neurons. We used the same microscopic techniques that demonstrate the presence of lipofuscin in mammalian neurons. Light microscopic studies demonstrated a regional correlation between autofluorescence, staining with Sudan Black, and the naturally occurring pigment in old R2s. Electron microscopic studies revealed the presence of large vacuolated and lamellated membrane‐bound bodies in the peripheral cytoplasm of old R2s, similar to those found in mammalian neurons. The bodies were located in the same region in which autofluorescence and Sudan Black staining were observed. Although the naturally occurring pigment accumulates with age, it acquires characteristics of lipofuscin pigment in the neurons of older sexually mature animals. The presence of these pigment characteristics can be used as an index of aging inAplysianeurons as they are in mammalian neurons.