INFLUENCE OF SALIVARY COMPONENTS AND EXTRACELLULAR POLYSACCHARIDE SYNTHESIS FROM SUCROSE ON ATTACHMENT OF STREPTOCOCCUS-MUTANS-6715 TO HYDROXYAPATITE SURFACES
INFLUENCE OF SALIVARY COMPONENTS AND EXTRACELLULAR POLYSACCHARIDE SYNTHESIS FROM SUCROSE ON ATTACHMENT OF STREPTOCOCCUS-MUTANS-6715 TO HYDROXYAPATITE SURFACES
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DOI：
10.1128/iai.18.2.514-523.1977
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发表时间：
1977-01-01
影响因子：
3.1
通讯作者：
GIBBONS, RJ
中科院分区：
文献类型：
--
作者：
CLARK, WB;GIBBONS, RJ
The adsorption of3H-labeledStreptococcus mutans6715 cells to disks of hydroxyapatite (HA) was studied. The number of streptococci that adsorbed was logarithmically related to the concentration of cells available up to at least 2 × 108per ml; equilibrium occurred within 45 min. Assay reliability was verified by direct scanning electron microscopic counts. Untreated HA disks exposed to buffered saline (PBS)-suspended streptococci at a concentration of 1.1 × 108per ml absorbed 3.2 × 106cells per cm2; approximately 3% of the surface area was, therefore, occupied by adsorbed organisms. The presence of adsorbed salivary components on HA reduced the number of attachingS. mutanscells by half. WhenS. mutanscells were suspended in saliva to mimic conditions existing in the mouth, the number of streptococci adsorbing to saliva-treated HA was reduced more than 30-fold compared to untreated HA. Approximately one-half of the streptococci adsorbed to untreated or to saliva-treated HA disks could be desorbed over a 4-h period with 0.067 M phosphate buffer.S. mutanscells exposed to sucrose to permit extracellular polysaccharide synthesis before or during adsorption attached in fewer numbers to both saliva-treated and untreated HA than PBS-treated organisms. WhenS. mutanscells adsorbed on untreated HA were exposed to sucrose, fewer organisms could be desorbed; thus, in situ polysaccharide synthesis promoted their more firm attachment to untreated HA. However, when saliva-suspended streptococci were adsorbed to saliva-treated HA surfaces, exposure to sucrose before or subsequent to adsorption did not promote more firm attachment. Evidently, the powerful adherence-inhibiting and desorptive effects of salivary components overshadowed any promoting effects attributable to glucan synthesis from sucrose. Similarly, no differences were noted in the desorption ofS. mutanscells from human teeth after exposure to sucrose, glucose, or PBS relative to a strain ofStreptococcus mitis(S. mitior). Thus, no evidence was obtained to support the hypothesis that glucan synthesis from sucrose was essential for, or promoted, the attachment ofS. mutanscells to HA surfaces exposed to saliva or to the smooth surfaces of human teeth.
