Apoptosis occurs after cerebral contusions in humans
Apoptosis occurs after cerebral contusions in humans
复制标题
DOI：
10.1097/00006123-200004000-00034
复制
发表时间：
2000-04-01
期刊：
影响因子：
4.8
通讯作者：
Smith, DR
中科院分区：
文献类型：
--
作者：
Ng, I;Yeo, TT;Smith, DR
目的：动物模型系统已经表明，头部创伤可以通过细胞凋亡过程诱导远离撞击部位的脑区域中的细胞死亡。我们试图确定是否有证据表明，在临床收集的材料从人类头部创伤患者的细胞凋亡，以及试图确定的途径，它可能occurrent.METHODS：31个连续标本的脑组织切除在紧急开颅手术疏散脑挫伤与质量效应进行了检查。在11个样本中检测到非坏死的挫伤周围组织。通过末端脱氧核苷酸转移酶介导的缺口末端标记法以及免疫组织化学检测凋亡相关基因p53、bcl-2和bax的表达来检测凋亡细胞的存在。所有患者中均检测到Bax表达，而6例患者中观察到bcl-2表达，末端脱氧核苷酸转移酶介导的缺口末端标记阳性细胞中注意到8例。观察到一例p53阳性免疫染色。bcl-2表达的患者有更好的生存率比没有bcl-2表达的患者被注意到（P = 0.01）。结论：虽然坏死似乎是主要发现在脑挫伤，这些结果支持的假设，即细胞凋亡发生在创伤性脑损伤后的患者，这可能有助于继发性损伤过程中所看到的头部损伤。诱导抗凋亡bcl-2的患者似乎有更好的预后。这可能对开发bcl-2同源物或bax抑制剂以防止细胞凋亡具有重要的临床意义。
OBJECTIVE: Animal model systems have shown that head trauma can induce cell death in regions of the brain away from the site of the impact via a process of apoptosis. We sought to determine whether there was evidence of cellular apoptosis in clinically collected materials from human head trauma patients, as well as to attempt to determine the pathway by which it may occur.METHODS: Thirty-one sequential specimens of brain tissue excised during emergency craniotomy for evacuation of cerebral contusions with mass effect were examined. Non-necrotic pericontusional tissues were detected in 11 samples. These were examined for the presence of apoptotic cells by the terminal deoxynucleotide transferase-mediated nick end labeling method as well as by immunohistochemistry to detect possible expression of the apoptosis-related genes p53, bcl-2, and bax.RESULTS: Bax expression was detected in all patients, whereas bcl-2 expression was noted in six patients, Terminal deoxynucleotide transferase-mediated nick end labeling-positive cells were noted in eight patients. One instance of p53-positive immunostaining was observed. Patients with bcl-2 expression had a better survival rate than patients in whom no bcl-2 expression was noted (P = 0.01).CONCLUSION: Although necrosis seemed to be the main finding in cerebral contusions, these results support the hypothesis that apoptosis does occur in patients after traumatic brain injury, and this may contribute to the secondary injury processes that are seen with head injury. Patients in whom anti-apoptotic bcl-2 is induced seem to have a better prognosis. This may have important clinical significance in the development of bcl-2 homologs or bax inhibitors to prevent apoptosis.