Isolation and characterization of genes encoding GRF transcription factors and GIF transcriptional coactivators in Maize (Zea mays L.)
Isolation and characterization of genes encoding GRF transcription factors and GIF transcriptional coactivators in Maize (Zea mays L.)
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DOI：
10.1016/j.plantsci.2008.08.002
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发表时间：
2008-12-01
期刊：
影响因子：
5.2
通讯作者：
Wang, Shou-Cai
中科院分区：
文献类型：
--
作者：
Zhang, Deng-Feng;Li, Bo;Wang, Shou-Cai
长期以来，人们一直认为，真核生物中基因表达的调控主要发生在转录水平。GRF(生长调节因子)和GIF(GRF相互作用因子)蛋白是公认的新型转录调节因子和共激活因子，它们形成蛋白质复合体，参与调控植物某些组织和器官的生长发育。本研究从玉米(Zea Mays L.)中获得了14个ZmGRF基因同源基因和3个ZmGIF基因同源基因。对其进行了鉴定和表征。14个GRF蛋白分为3个亚家族。C亚家族的ZmGRF基因主要在幼穗中表达。此外，与野生型拟南芥相比，ZmGRF11-ZmGIF2和ZmGRF2-ZmGIF3的过表达导致了花序抽苔的延迟，但促进了花序茎的生长。因此，我们推测这四个基因在玉米幼穗组织中优先表达，可能在玉米幼穗的生长发育中发挥重要作用，并可能有助于玉米产量的提高。(C)2008爱思唯尔爱尔兰有限公司。保留所有权利。
It has been a long-held view that regulation of gene expression in eukaryotes occurs predominantly at the transcriptional level. GRF (Growth-regulating factor) and GIF (GRF-interacting factor) proteins are putative novel transcriptional regulators and coactivators which form protein complexes and are involved in regulating the growth and development of certain tissues and/or organs in plants. In this study, 14 homologs of ZmGRF genes and 3 homologs of ZmGIF genes from maize (Zea mays L.) were identified and characterized. Fourteen GRF proteins were divided into three subfamilies. ZmGRF genes of C subfamily were mainly expressed in immature ears. Furthermore, overexpression of both ZmGRF11-ZmGIF2 and ZmGRF2-ZmGIF3 resulted in delayed bolting but accelerating the growth of inflorescence stem when compared to wild-type Arabidopsis thaliana. As a result, we postulated that these four genes which expressed preferentially in immature ear tissues in maize might play important roles in the growth and development of ears and might contribute to improving corn yields. (c) 2008 Elsevier Ireland Ltd. All rights reserved.