Retinaldehyde, a potent inhibitor of gap junctional intercellular communication.
Retinaldehyde, a potent inhibitor of gap junctional intercellular communication.
复制标题
视黄醛，间隙连接细胞间通讯的有效抑制剂。
DOI：
10.1080/15419060490471784
复制
发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Sitaramayya,Ari
中科院分区：
文献类型：
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作者：
Pulukuri,Sadhona;Sitaramayya,Ari
视黄醛和视黄酸是维生素A的衍生物，并且视黄醛是合成视黄酸的前体，视黄酸是众所周知的间隙连接细胞间通讯抑制剂。在这项研究中，我们提出了这样一个问题，即视黄醇是否对缝隙连接有类似的作用。间隙连接细胞间通讯的测定刮加载和预加载染料转移方法，并进行了研究，主要是在培养的肝上皮细胞。发现视黄醛是比视黄酸（在30 μM时染料转移减少50%）和大黄酸（在65 μM时染料转移减少50%）更有效的抑制剂（在2.8 μM时染料转移减少50%）。视黄醇的11-顺式和全反式形式同样有效。视黄醛抑制阴离子荧光黄和阳离子神经生物素的染料转移。在50 μM浓度下，视黄醇的抑制作用在不到2分钟内就出现了，但与报道的维甲酸不同，恢复较慢，尽管完全恢复。除肝上皮细胞外，视黄醇还抑制透镜上皮细胞、视网膜色素上皮细胞和视网膜神经节细胞的缝隙连接通讯。
Retinaldehyde and retinoic acid are derivatives of vitamin A, and retinaldehyde is the precursor for the synthesis of retinoic acid, a well-known inhibitor of gap junctional intercellular communication. In this investigation, we asked the question if retinaldehyde has similar effects on gap junctions. Gap junctional intercellular communication was measured by scrape-loading and preloading dye-transfer methods, and studies were carried out mainly on cultured liver epithelial cells. Retinaldehyde was found to be a more potent inhibitor (dye transfer reduced by 50% at 2.8 μM) than retinoic acid (dye transfer reduced by 50% at 30 μM) and glycyrrhetinic acid (dye transfer reduced by 50% at 65 μM). Both the 11-cis and all-trans forms of retinaldehyde were equally effective. Retinaldehyde inhibited dye transfer of both anionic Lucifer yellow and cationic Neurobiotin. Inhibition by retinaldehyde developed in less than two minutes at 50 μM, but unlike the reported case with retinoic acid, recovery was slower, though full. In addition to liver epithelial cells, retinaldehyde inhibited gap junctional communication in lens epithelial cells, retinal pigment epithelial cells and retinal ganglion cells.