Expression of angiotensin II receptors in human left and right atrial tissue in atrial fibrillation with and without underlying mitral valve disease
Expression of angiotensin II receptors in human left and right atrial tissue in atrial fibrillation with and without underlying mitral valve disease
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DOI：
10.1016/j.jacc.2003.07.014
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发表时间：
2003-11-19
影响因子：
24
通讯作者：
Dhein, S
中科院分区：
文献类型：
--
作者：
Boldt, A;Wetzel, U;Dhein, S
目的 与窦性心律 (SR) 相比，我们假设孤立性心房颤动 (AF) 和 AF 合并潜在二尖瓣疾病 (MVD) 患者的血管紧张素 II 受体亚型表达发生变化。 背景 心房颤动是一种与电和结构重塑相关的进行性疾病。血管紧张素 II (ANGII) 参与心肌重塑过程。 ANGII 的作用由 ANGII 受体亚型 1 和 2（AT(1) 和 AT(2)）介导。 方法 左心房 (LA) 和右心房 (RA) 组织样本取自患有或不患有潜在 MVD 的 AF 或 SR 患者。通过定量蛋白质印迹技术测量 AT(1) 和 AT(2) 蛋白水平。 结果 与 SR 相比，AF 患者（所有形式）LA 中的 AT, 蛋白水平显着升高 (p < 0.05)，而 AT(2) 表达没有显着改变。亚组比较显示，阵发性 AF 和伴有或不伴有 MVD 的慢性 AF 中 AT(1) 都有相似的增加。此外，对 RA ANGII 受体亚型的研究并未显示 AF 患者与 SR 患者的 AT(1) 或 AT(2) 存在任何显着变化。潜在的 MVD 并未显着影响 LA 中 AT(2) 受体亚型的表达。 结论 心房颤动与 LA 中 AT(1) 的上调相关，但与 RA 中无关，并且似乎不影响心房中 AT(2) 的表达。由于我们发现 LA 中 AT(1) 的表达增强，因此我们得出结论，AT 可能参与 LA 中 AF 的发病机制。 (C) 2003 年由美国心脏病学会基金会资助。
OBJECTIVES We postulated a change of angiotensin II receptor subtype expression in patients with lone atrial fibrillation (AF) and AF with underlying mitral valve disease (MVD) both compared with sinus rhythm (SR).BACKGROUND Atrial fibrillation is a progressive disease associated with electrical and structural remodeling. Angiotensin II (ANGII) is involved in the process of myocardial remodeling. Actions of ANGII are mediated by ANGII receptor subtypes 1 and 2 (AT(1) and AT(2)).METHODS Left atrial (LA) and right atrial (RA) tissue samples were obtained from patients with AF or SR with or without underlying MVD. The AT(1) and AT(2) protein levels were measured by quantitative Western blotting techniques.RESULTS The AT, protein level in the LA was significantly increased in patients with AF (all forms) compared with SR (p < 0.05), whereas AT(2) expression was not significantly altered. Comparison of the subgroups revealed a similar increase of AT(1) in both paroxysmal AF and chronic AF with or without MVD. Additionally, investigations of ANGII receptor subtypes in the RA did not exhibit any significant changes either in AT(1) or in AT(2) in patients with AF versus SR. Underlying MVD did not significantly affect AT(2) receptor subtype expression in LA.CONCLUSIONS Atrial fibrillation is associated with an up-regulation of AT(1) in LA, but not in RA, and did not appear to influence the AT(2) expression in the atrium. Because we found an enhanced expression of AT(1) in the LA, we conclude that AT, might be involved in the pathogenesis of AF in the LA. (C) 2003 by the American College of Cardiology Foundation.