The protective effect of intermittent calorie restriction on mammary tumorigenesis is not compromised by consumption of a high fat diet during refeeding.
The protective effect of intermittent calorie restriction on mammary tumorigenesis is not compromised by consumption of a high fat diet during refeeding.
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间歇性热量限制对乳腺肿瘤发生的保护作用不会因重新喂养期间高脂肪饮食的消耗而受到损害。
DOI：
10.1007/s10549-013-2464-7
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发表时间：
2013
影响因子：
3.8
通讯作者：
Cleary,MargotP
中科院分区：
文献类型：
--
作者：
Rogozina,OlgaP;Nkhata,KataiJ;Nagle,EmilyJ;Grande,JosephP;Cleary,MargotP
之前我们报道过间歇性卡路里限制（ICR）比慢性卡路里限制（CCR）更能预防乳腺肿瘤（MTs）。本研究评估了ICR方案中再饲喂期间脂肪摄入量增加的影响。MMTV-TGF-α雌性小鼠被分配到三组中的一组：随意喂食（n= 45），并自由获得中等高脂肪饮食（22%脂肪卡路里）；ICR (n= 45) 50%卡路里限制3周，然后3周100% AL摄入量；和CCR （n= 45）分别饲喂75%的AL小鼠，与ICR小鼠每6周的周期相匹配。根据在这些时间间隔内获得的数据，将ICR小鼠进一步指定为ICR- restricted或ICR- refed。所有小鼠摄入的绝对脂肪量相同。随访小鼠，评估MT发生率、体重和血清IGF-1、IGFBP3、瘦素和脂联素水平，直至79周龄（最后3周限饲结束）或82周龄（最后3周复饲结束）。与AL小鼠的57.5周相比，CCR小鼠（74周）和ICR小鼠（82周）的MT检测年龄显著延长。AL、ICR和CCR小鼠的MT发生率分别为66.7%、4.4%和52.3%。与AL、CCR和ICR-Refed小鼠相比，ICR-Restricted小鼠的乳腺和脂肪垫重量在卡路里限制50%后显著减少。在研究过程中，icr限制小鼠的IGF-1和瘦素水平也趋于降低，而脂联素没有与AL、CCR和ICR-Refed小鼠进行比较。在icr受限的小鼠中，脂联素：瘦素比例在50%限制后始终较高。在任何组中，IGF-1、瘦素或脂联素与MTs的存在都没有关系。因此，限制热量摄入的方式会影响热量限制的保护作用，而与高脂肪摄入无关。
Previously we reported that intermittent calorie restriction (ICR) provided greater prevention of mammary tumors (MTs) than chronic calorie restriction (CCR). Here the impact of increased fat intake during refeeding in an ICR protocol was evaluated. MMTV-TGF-α female mice were assigned to one of three groups: ad libitum (AL) fed (n= 45) with free access to a moderately high fat diet (22 % fat calories); ICR (n= 45) 50 % calorie restricted for 3-week intervals followed by 3 weeks of 100 % of AL intake; and CCR (n= 45) fed 75 % of AL mice, matching each 6-week cycle of ICR mice. ICR mice were further designated as ICR-Restricted or ICR-Refed for data obtained during these intervals. All mice consumed the same absolute amount of dietary fat. Mice were followed to assess MT incidence, body weight and serum IGF-1, IGFBP3, leptin and adiponectin levels until 79 (end of final 3-week restriction) or 82 (end of final 3-weeks refeeding) weeks of age. Age of MT detection was significantly extended for CCR (74 weeks) and ICR (82 weeks) mice, compared to 57.5 weeks for AL mice. MT incidence for AL, ICR and CCR mice was 66.7, 4.4, and 52.3 %, respectively. Mammary and fat pad weights were reduced significantly following 50 % calorie restriction in ICR-Restricted mice compared to AL, CCR and ICR-Refed mice. IGF-1 and leptin levels also tended to be reduced in ICR-Restricted mice over the course of the study while adiponectin was not compared to AL, CCR, and ICR-Refed mice. The adiponectin:leptin ratio was consistently higher following 50 % restriction in ICR-Restricted mice. There was no relationship of IGF-1, leptin, or adiponectin with the presence of MTs in any groups. Thus the manner in which calories are restricted impacts the protective effect of calorie restriction independently of high fat intake.
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